An HcpR paralog ofDesulfovibrio gigas provides protection against nitrosative stress

Abstract

Desulfovibrio gigas belongs to the group of sulfate reducing bacteria (SRB). These ubiquitous and metabolically versatile microorganisms are often exposed to reactive nitrogen species (RNS). Nonetheless, the mechanisms and regulatory elements involved in nitrosative stress protection are still poorly understood. The transcription factor HcpR has emerged as a putative regulator of nitrosative stress response among anaerobic bacteria. HcpR is known to orchestrate the expression of the hybrid cluster protein gene,hcp, proposed to be involved in cellular defense against RNS. According to phylogenetic analyses, the occurrence ofhcpR paralog genes is a common feature among severalDesulfovibrio species. Within theD. gigas genome we have identified two HcpR‐related sequences. One of these sequences,hcpR1, was found in the close vicinity of thehcp gene and this finding prompted us to proceed with its functional characterization. We observed that the growth of aD. gigas strain lackinghcpR1 is severely impaired under nitrosative stress. Anin silico search revealed several putative targets of HcpR1 that were experimentally validated. The fact that HcpR1 regulates several genes encoding proteins involved in nitrite and nitrate metabolism, together with the sensitive growth phenotype to NO displayed by anhcpR1 mutant strain, strongly supports a relevant role of this factor under nitrosative stress. Moreover, the finding that severalDesulfovibrio species possess HcpR paralogs, which have been transmitted vertically in the evolution and diversification of the genus, suggests that these sequences may confer adaptive or survival advantage to these organisms, possibly by increasing their tolerance to nitrosative stress.

Keywords

• Desulfovibrio
• HcpR
• Nitrosative stress
• Sulfate reducing bacteria
• Transcription regulation
• Molecular phylogeny