Anticancer and anti-angiogenic activities of mannooligosaccharides extracted from coconut meal on colorectal carcinoma cells in vitro

Patthra Pason; Chakrit Tachaapaikoon; Waralee Suyama; Rattiya Waeonukul; Rong Shao; Molin Wongwattanakul; Temduang Limpaiboon; Chirapond Chonanant; Nipaporn Ngernyuang

Toxicology Reports (Jun 2024)

Anticancer and anti-angiogenic activities of mannooligosaccharides extracted from coconut meal on colorectal carcinoma cells in vitro

Patthra Pason,
Chakrit Tachaapaikoon,
Waralee Suyama,
Rattiya Waeonukul,
Rong Shao,
Molin Wongwattanakul,
Temduang Limpaiboon,
Chirapond Chonanant,
Nipaporn Ngernyuang

Affiliations

Patthra Pason: School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok 10150, Thailand; Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkok 10150, Thailand
Chakrit Tachaapaikoon: School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok 10150, Thailand; Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkok 10150, Thailand
Waralee Suyama: School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok 10150, Thailand; Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkok 10150, Thailand
Rattiya Waeonukul: School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok 10150, Thailand; Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkok 10150, Thailand
Rong Shao: Shanghai Key Laboratory for Gallbladder Cancer-Related Gastroenterological Diseases, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200089, China
Molin Wongwattanakul: Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Science, Khon Kaen University, Khon Kaen 40002, Thailand
Temduang Limpaiboon: Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Science, Khon Kaen University, Khon Kaen 40002, Thailand
Chirapond Chonanant: Department of Medical Technology, Faculty of Allied Health Science, Burapha University, Chonburi 20131, Thailand
Nipaporn Ngernyuang: Chulabhorn International College of Medicine, Thammasat University, Pathum Thani 12120, Thailand; Corresponding author.

Journal volume & issue: Vol. 12
pp. 82 – 90

Abstract

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Colorectal carcinoma (CRC) is one of the most common malignancies, though there are no effective therapeutic regimens at present. This study aimed to investigate the inhibitory effects of mannooligosaccharides extracted from coconut meal (CMOSs) on the proliferation and migration of human colorectal cancer HCT116 cells in vitro. The results showed that CMOSs exhibited significant inhibitory activity against HCT116 cell proliferation in a concentration-dependent manner with less cytotoxic effects on the Vero normal cells. CMOSs displayed the ability to increase the activation of caspase-8, –9, and –3/7, as well as the generation of reactive oxygen species (ROS). Moreover, CMOSs suppressed HCT116 cell migration in vitro. Interestingly, treatment of human microvascular endothelial cells (HMVECs) with CMOSs resulted in the inhibition of cell proliferation, cell migration, and capillary-like tube formation, suggesting its anti-vascular angiogenesis. In summary, the results of this study indicate that CMOSs could be a valuable therapeutic candidate for CRC treatment.

Published in Toxicology Reports

ISSN: 2214-7500 (Online)
Publisher: Elsevier
Country of publisher: Ireland
LCC subjects: Medicine: Public aspects of medicine: Toxicology. Poisons
Website: http://www.journals.elsevier.com/toxicology-reports/

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