HPLC-ESI- MS/MS analysis of beet (Beta vulgaris) leaves and its beneficial properties in type 1 diabetic rats

Eman A. Abd El-Ghffar; Nesrine M. Hegazi; Hamada H. Saad; Mohamed M. Soliman; Mohamed A. El-Raey; Safia M. Shehata; Alaa Barakat; Aziz Yasri; Mansour Sobeh

Biomedicine & Pharmacotherapy (Dec 2019)

HPLC-ESI- MS/MS analysis of beet (Beta vulgaris) leaves and its beneficial properties in type 1 diabetic rats

Eman A. Abd El-Ghffar,
Nesrine M. Hegazi,
Hamada H. Saad,
Mohamed M. Soliman,
Mohamed A. El-Raey,
Safia M. Shehata,
Alaa Barakat,
Aziz Yasri,
Mansour Sobeh

Affiliations

Eman A. Abd El-Ghffar: Biology Department, Faculty of Science, Taibah University, Yanbu Branch, PO Box 46526, Saudi Arabia; Zoology Department, Faculty of Science, Ain Shams University, Cairo, PO Box 11566, Egypt
Nesrine M. Hegazi: Phytochemistry and Plant Systematics Department, Division of Pharmaceutical Industries, National Research Centre, Cairo, PO Box 12622, Egypt; Corresponding author.
Hamada H. Saad: Phytochemistry and Plant Systematics Department, Division of Pharmaceutical Industries, National Research Centre, Cairo, PO Box 12622, Egypt; Department of Pharmaceutical Biology, Pharmaceutical Institute, Eberhard Karls University of Tübingen, Tübingen, PO Box 72074, Germany
Mohamed M. Soliman: Zoology Department, Faculty of Science, Ain Shams University, Cairo, PO Box 11566, Egypt; Biology Department, Faculty of Science, Jazan University, KSA PO Box 2097, Saudi Arabia
Mohamed A. El-Raey: Phytochemistry and Plant Systematics Department, Division of Pharmaceutical Industries, National Research Centre, Cairo, PO Box 12622, Egypt
Safia M. Shehata: Clinical Pathology Department, Ain Shams University Hospitals, Cairo, PO Box 11566, Egypt
Alaa Barakat: Department of Biochemistry and Biotechnology, Faculty of Pharmacy, Heliopolis University, Cairo, PO Box11361, Egypt
Aziz Yasri: AgroBioSciences Research Division, Mohammed VI Polytechnic University, Lot 660–Hay MoulayRachid, 43150 Ben-Guerir, Morocco
Mansour Sobeh: AgroBioSciences Research Division, Mohammed VI Polytechnic University, Lot 660–Hay MoulayRachid, 43150 Ben-Guerir, Morocco; Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, 69120, Heidelberg, Germany; Corresponding author at: AgroBioSciences Research Division, Mohammed VI Polytechnic University, Lot 660–Hay MoulayRachid, 43150 Ben-Guerir, Morocco.

Journal volume & issue: Vol. 120

Abstract

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The phenolic profile of the leaves of Beta vulgaris subspecies vulgaris variety rubra was investigated by high-performance liquid chromatography (HPLC) coupled to electrospray ionization high resolution mass spectrometric (ESI-HRMS-MS) detection. Mass spectrometry-based molecular networking was employed to dereplicate the known compounds. Twelve known compounds, seven of which are previously undescribed as constituents in the B. vulgaris leaves were dereplicated and assigned with various levels of identification confidence. The ameliorative effects of the aqueous methanolic extract of the leaves were assessed against alloxan induced diabetic rats. It was found that the extract significantly decreased (p < 0.001) serum glucose, lipid profile, ALT, AST, TNF-α, IL-1β, IL-6, and hepatic MDA levels; and significantly increased (p < 0.001) hepatic TAO and GSH; and down-regulated the expression of hepatic NF-κB versus the untreated diabetic groups, in a dose-dependent manner. In molecular docking, all identified compounds exhibited good glide score against the PPAR-ɣ target, confirming the in vivo observed activities. In conclusion, B. vulgaris has immunomodulatory / antioxidant effects that could be helpful in slowing the progression of diabetic complications.

Published in Biomedicine & Pharmacotherapy

ISSN: 0753-3322 (Print); 1950-6007 (Online)
Publisher: Elsevier
Country of publisher: France
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: https://www.journals.elsevier.com/biomedicine-and-pharmacotherapy

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