Transcription Regulation of Anthocyanins and Proanthocyanidins Accumulation by Bagging in ‘Ruby’ Red Mango: An RNA-seq Study
Wencan Zhu,
Hongxia Wu,
Chengkun Yang,
Xiaowen Wang,
Bin Shi,
Bin Zheng,
Xiaowei Ma,
Minjie Qian,
Aiping Gao,
Kaibing Zhou
Affiliations
Wencan Zhu
Sanya Nanfan Research Institute & Key Laboratory of Quality Regulation of Tropical Horticultural Crop in Hainan Province, Hainan University, Haikou 570228, China
Hongxia Wu
Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524013, China
Chengkun Yang
Sanya Nanfan Research Institute & Key Laboratory of Quality Regulation of Tropical Horticultural Crop in Hainan Province, Hainan University, Haikou 570228, China
Xiaowen Wang
Sanya Nanfan Research Institute & Key Laboratory of Quality Regulation of Tropical Horticultural Crop in Hainan Province, Hainan University, Haikou 570228, China
Bin Shi
Sanya Nanfan Research Institute & Key Laboratory of Quality Regulation of Tropical Horticultural Crop in Hainan Province, Hainan University, Haikou 570228, China
Bin Zheng
Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524013, China
Xiaowei Ma
Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524013, China
Minjie Qian
Sanya Nanfan Research Institute & Key Laboratory of Quality Regulation of Tropical Horticultural Crop in Hainan Province, Hainan University, Haikou 570228, China
Aiping Gao
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences & Ministry of Agriculture Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Haikou 571100, China
Kaibing Zhou
Sanya Nanfan Research Institute & Key Laboratory of Quality Regulation of Tropical Horticultural Crop in Hainan Province, Hainan University, Haikou 570228, China
The biosynthesis of anthocyanins and proanthocyanidins (PAs), components of two main flavonoids in plants, is regulated by environmental factors such as light. We previously found that bagging significantly repressed the biosynthesis of anthocyanins in red ‘Ruby’ mango fruit peel, but induced the accumulation of PAs. However, the molecular mechanism remains unclear. In the current study, transcriptome sequencing was used for screening the essential genes responsible for the opposite accumulation pattern of anthocyanins and PAs by bagging treatment. According to weighted gene co-expression network analysis (WGCNA), structural genes and transcription factors highly positively correlated to anthocyanins and PAs were identified. One flavanone 3-hydroxylase (F3H) and seven structural genes, including one chalcone synthase (CHS), one flavonoid 3’-hydroxylase (F3’H), one anthocyanidin synthesis (ANS), three leucoanthocyanidin reductase (LARs), and one UDP glucose: flavonoid 3-O-glucosyltransferase (UFGT), are crucial for anthocyanin and PA biosynthesis, respectively. In addition to MYB and bHLH, ERF, C2H2, HD-ZIP, and NAC are important transcription factors that participate in the regulation of anthocyanin and PA biosynthesis in ‘Ruby’ mango fruit peel by bagging treatment. Our results are helpful for revealing the transcription regulation mechanism of light-regulated mango anthocyanin and PA biosynthesis, developing new technologies for inducing flavonoid biosynthesis in mangos, and breeding mango cultivars containing high concentrations of flavonoids.
