Alleviating D-Galactose-Induced Aging in Mice by Modulating Gut-Liver Axis Using <i>Lactiplantibacillus plantarum</i> TY-Y10
Shaoqi Shi,
Xiaoxia Li,
Feng Zhang,
Zhengqiang Jiang,
Jing Wang,
Liang Zhao,
Juan Chen,
Xi Shu,
Bing Fang,
Ping Liu,
Jingjing He,
Shaoyang Ge,
Fuqing Wang,
Jie Guo,
Yixuan Li,
Jie Luo,
Ran Wang
Affiliations
Shaoqi Shi
Key Laboratory of Functional Dairy, Department of Nutrition and Health, Co-Constructed by Ministry of Education and Beijing Government, China Agricultural University, Beijing 100190, China
Xiaoxia Li
Key Laboratory of Functional Dairy, Department of Nutrition and Health, Co-Constructed by Ministry of Education and Beijing Government, China Agricultural University, Beijing 100190, China
Feng Zhang
Chongqing Key Laboratory for Industry and Informatization of Probiotic Fermentation Technology in Dairy Products, Chongqing Tianyou Dairy Co., Ltd., Chongqing 401120, China
Zhengqiang Jiang
Key Laboratory of Food Bioengineering (China National Light Industry), College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
Jing Wang
Chongqing Key Laboratory for Industry and Informatization of Probiotic Fermentation Technology in Dairy Products, Chongqing Tianyou Dairy Co., Ltd., Chongqing 401120, China
Liang Zhao
Research Center for Probiotics, China Agricultural University, Beijing 100083, China
Juan Chen
Key Laboratory of Functional Dairy, Department of Nutrition and Health, Co-Constructed by Ministry of Education and Beijing Government, China Agricultural University, Beijing 100190, China
Xi Shu
Chongqing Key Laboratory for Industry and Informatization of Probiotic Fermentation Technology in Dairy Products, Chongqing Tianyou Dairy Co., Ltd., Chongqing 401120, China
Bing Fang
Food Laboratory of Zhongyuan, Luohe 461103, China
Ping Liu
Key Laboratory of Functional Dairy, Department of Nutrition and Health, Co-Constructed by Ministry of Education and Beijing Government, China Agricultural University, Beijing 100190, China
Jingjing He
Research Center for Probiotics, China Agricultural University, Beijing 100083, China
Shaoyang Ge
Hebei Engineering Research Center of Animal Product, Sanhe 065200, China
Fuqing Wang
Tibet Tianhong Science and Technology Co., Ltd., Lhasa 850000, China
Jie Guo
Research Center for Probiotics, China Agricultural University, Beijing 100083, China
Yixuan Li
Research Center for Probiotics, China Agricultural University, Beijing 100083, China
Jie Luo
College of Food Science and Technology, Hunan Agricultural University, Changsha 410114, China
Ran Wang
Key Laboratory of Functional Dairy, Department of Nutrition and Health, Co-Constructed by Ministry of Education and Beijing Government, China Agricultural University, Beijing 100190, China
Oxidative stress is closely linked to aging. Probiotics, whether viable or heat-inactivated, have shown antioxidant properties; however, their effect and mechanism of action in reducing oxidative stress during aging remains underexplored. This study examined the effects of viable and heat-inactivated Lactiplantibacillus plantarum TY-Y10 (L. plantarum TY-Y10) on D-galactose (D-gal)-induced aging in mice, aiming to uncover potential anti-aging mechanisms. Mice were induced to age with D-gal injections, then treated with sodium ascorbate (positive control) or varying doses of L. plantarum TY-Y10 for eight weeks. After treatment, oxidative stress markers, gut microbiota, and liver health were analyzed. Results showed that L. plantarum TY-Y10 decreased malondialdehyde (MDA) and inflammatory markers while increasing antioxidant levels (glutathione, superoxide dismutase, catalase and glutathione peroxidase). Liver damage was reduced, and expression of Nrf2 and related antioxidant enzymes improved. Additionally, L. plantarum TY-Y10 enhanced the abundance of short-chain fatty acid-producing bacteria, boosting fecal short-chain fatty acid levels. In short, both viable and heat-inactivated L. plantarum TY-Y10 mitigated oxidative stress in aging mice by modulating gut microbiota and activating liver antioxidant pathways through the gut-liver axis.
