Lipidomic Analysis of Caenorhabditis elegans Embryos
Hung-Chi Yang,
Cheng-Yu Hung,
Yi-Yun Pan,
Szecheng Lo,
Daniel Chiu
Affiliations
Hung-Chi Yang
Healthy Aging Research Center, Chang Gung University, Kwei-San, Tao-Yuan, TaiwanDepartment of Medical Laboratory Science and Biotechnology, Yuanpei University of Medical Technology, Hsinchu, Taiwan
Cheng-Yu Hung
Healthy Aging Research Center, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan
Yi-Yun Pan
Department of Medical Biotechnology and Laboratory Sciences, College of Medicine, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan
Szecheng Lo
Department of Biomedical Sciences, College of Medicine, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan
Daniel Chiu
Healthy Aging Research Center, Chang Gung University, Kwei-San, Tao-Yuan, TaiwanDepartment of Medical Biotechnology and Laboratory Sciences, College of Medicine, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan, Department of Pediatric Hematology/Oncology, Linkou Chang Gung Memorial Hospital, Tao-Yuan, Taiwan, Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, Kwei-San, Tao-Yuan, Taiwan
Metabolomic is an emerging field of system biology. Lipidomic, a branch of metabolomic, aims to characterize lipophilic metabolites in biological systems. Caenorhabditis elegans (C. elegans) is a genetically tractable and versatile animal model for novel discovery of lipid metabolism. In addition, C. elegans embryo is simple and homogeneous. Here, we demonstrate detailed procedures of C. elegans culture, embryo isolation, lipid extraction and metabolomic data analysis.
