Pteridines (Aug 1989)

Identification of Folates by Iodine Oxidation at Acid, Neutral and Alkaline pH

  • Coppell A. D.,
  • Leeming R. J.,
  • Blair J. A.

DOI
https://doi.org/10.1515/pteridines.1989.1.3.155
Journal volume & issue
Vol. 1, no. 3
pp. 155 – 157

Abstract

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Oxidation by iodine at pH 1.5, pH 7.0 with and without catalase and at pH 12.5, differentiated folic acid, 5- methyltetrahydrofolic acid, 10-formylfolic acid, 10-formyltetrahydrofolic acid and 5-formyltetrahydrofolic acid, when the products were assayed with Lactobacillus casei. This method is proposed as an alternative to differential microbiological assay for identifying folates.