Screening Test for Detection of Leptinotarsa decemlineata (Say) Sensitivity to Insecticides

Abstract

In 2009, the sensitivity of 15 field populations of Colorado potato beetle (Leptinotarsadecemlineata Say.) - CPB was assessed to chlorpyrifos, cypermethrin, thiamethoxam and fipronil,four insecticides which are mostly used for its control in Serbia. Screening test that allows rapidassessment of sensitivity of overwintered adults to insecticides was performed. Insecticideswere applied at label rates, and two, five and 10 fold higher rates by soaking method (5 sec).Mortality was assessed after 72h. From 15 monitored populations of CPB, two were sensitiveto label rate of chlorpyrifos, one was slightly resistant, 11 were resistant and one populationwas highly resistant. Concerning cypermethrin, two populations were sensitive, two slightlyresistant, five were resistant and six highly resistant. Highly sensitive to thiamethoxam labelrate were 12 populations, while three were sensitive. In the case of fipronil applied at label rate,two populations were highly sensitive, six sensitive, one slightly resistant and six were resistant.The application of insecticides at higher rates (2, 5 and 10 fold), that is justified only in bioassays,provided a rapid insight into sensitivity of field populations of CPB to insecticides.

Keywords

• Leptinotarsa decemlineata-CPB
• Insecticides
• Sensitivity
• Resistance
• Monitoring