Solid-Phase Immunoassay of Polystyrene-Encapsulated Semiconductor Coreshells for Cardiac Marker Detection

Journal of Nanomaterials. 2012;2012 DOI 10.1155/2012/693575

 

Journal Homepage

Journal Title: Journal of Nanomaterials

ISSN: 1687-4110 (Print); 1687-4129 (Online)

Publisher: Hindawi Publishing Corporation

LCC Subject Category: Technology: Technology (General)

Country of publisher: Egypt

Language of fulltext: English

Full-text formats available: PDF, HTML, ePUB

 

AUTHORS

Sanghee Kim (Department of Biomedical Engineering, College of Health Science, Korea University, San 1, Jeongneung-3-dong, Seongbuk-gu, Seoul 136-703, Republic of Korea)
Jaetae Seo (Department of Physics, Advanced Center for Laser Science and Spectroscopy, Hampton University, Hampton, VA 23668, USA)
Roopchan Ramdon (Department of Physics, Advanced Center for Laser Science and Spectroscopy, Hampton University, Hampton, VA 23668, USA)
Hyeon-Bong Pyo (BioMed Team, Electronics and Telecommunications Research Institute, 131 Gajeong-Dong, Daejeon 305-700, Republic of Korea)
Kyuho Song (Devices & Materials Lab. LG Electronics, 16 Woomyeon-dong, Secho-gu, Seoul 137-724, Republic of Korea)
Byoung Hun Kang (Department of Mechanical Design Engineering, Korea Polytechnic University, 2121 Jungwang-Dong, Kyonggi-Do, Siheung-City 429-793, Republic of Korea)

EDITORIAL INFORMATION

Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 16 weeks

 

Abstract | Full Text

A solid-phase immunoassay of polystyrene-encapsulated semiconductor nanoparticles was demonstrated for cardiac troponin I (cTnI) detection. CdSe/ZnS coreshells were encapsulated with a carboxyl-functionalized polystyrene nanoparticle to capture the target antibody through a covalent bonding and to eliminate the photoblinking and toxicity of semiconductor luminescent immunosensor. The polystyrene-encapsulated CdSe/ZnS fluorophores on surface-modified glass chip identified cTnI antigens at the level of ~ng/mL. It was an initial demonstration of diagnostic chip for monitoring a cardiovascular disease.