Differentiation of bone marrow mesenchymal stem cells into cardiomyocytes with FGF-2 and Panax notoginseng saponins

DONG Zihan; DONG Zihan; WANG Haiping; WANG Haiping; LYU Yang

doi:10.16016/j.2097-0927.202404018

陆军军医大学学报 (Nov 2024)

Differentiation of bone marrow mesenchymal stem cells into cardiomyocytes with FGF-2 and Panax notoginseng saponins

DONG Zihan,
DONG Zihan,
WANG Haiping,
WANG Haiping,
LYU Yang

Affiliations

DONG Zihan: Department of Histology and Embryology, School of Basic Medical Sciences, Hebei North University, Zhangjiakou, Hebei Province, 075000, China
DONG Zihan: Laboratory of Stem Cell and Reproductive Biology, Hebei North University, Zhangjiakou, Hebei Province, 075000, China
WANG Haiping: Department of Histology and Embryology, School of Basic Medical Sciences, Hebei North University, Zhangjiakou, Hebei Province, 075000, China
WANG Haiping: Laboratory of Stem Cell and Reproductive Biology, Hebei North University, Zhangjiakou, Hebei Province, 075000, China
LYU Yang: Department of Histology and Embryology, School of Basic Medical Sciences, Hebei North University, Zhangjiakou, Hebei Province, 075000, China

DOI: https://doi.org/10.16016/j.2097-0927.202404018
Journal volume & issue: Vol. 46, no. 21
pp. 2415 – 2423

Abstract

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Objective To investigate the effects of basic fibro-blast growth factor (FGF-2) and Panax notoginseng saponins (PNS) alone or combined together on the differentiation of bone marrow mesenchymal stem cells (BMSCs) into cardiomyocytes (CMs) and the mechanism of action of PNS and FGF-2 in the differentiation. Methods BMSCs were isolated from 3-week-old SD rats (20~30 g) by whole bone marrow adherence, and then the purity of BMSCs was identified by flow cytometry. After the purity was qualified, PNS and FGF-2 alone or in combination, or combined with LY294002 (phosphatidylinositol 3 kinase inhibitor), were used to induce BMSCs to differentiate. According to the corresponding culture time, the cells were divided into control group, PNS group, FGF-2 group, and PNS+FGF-2 group. The expression levels of Desmin and Cx43 after induction were detected by immunohistochemical assay. Western blotting was used to measure the expression levels of myocardium-specific proteins, related pathway proteins, and apoptosis-related proteins. RT-qPCR was conducted to detect the expression of myocardial transcription factors. Results The expression levels of myocardium-specific proteins and genes were increased in each single induction group (PNS group and FGF-2 group) and combined induction group (PNS+FGF-2 group, P < 0.05), and the highest expression levels were observed in the combined group. PNS and FGF-2 combined with LY294002 decreased the expression levels of myocardium-specific proteins and genes, decreased the phosphorylation of pathway-related protein Akt, decreased the level of Bcl-2, increased the level of Bax, and decreased the ratio of Bcl-2/Bax when compared with the 2 single induction groups (P < 0.05). Conclusion Both PNS and FGF-2 can induce BMSCs to differentiate into CMs after induction, and the combined induction is more effective in the differentiation. The PI3K/Akt signaling pathway is involved in the differentiation process by PNS and FGF-2, and PNS and FGF-2 play a role in reducing apoptosis.

Published in 陆军军医大学学报

ISSN: 2097-0927 (Print)
Publisher: Editorial Office of Journal of Army Medical University
Country of publisher: China
LCC subjects: Medicine: Medicine (General)
Website: http://aammt.tmmu.edu.cn/

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