Centromeres are maintained by fastening CENP-A to DNA and directing an arginine anchor-dependent nucleosome transition

Lucie Y. Guo; Praveen Kumar Allu; Levani Zandarashvili; Kara L. McKinley; Nikolina Sekulic; Jennine M. Dawicki-McKenna; Daniele Fachinetti; Glennis A. Logsdon; Ryan M. Jamiolkowski; Don W. Cleveland; Iain M. Cheeseman; Ben E. Black

doi:10.1038/ncomms15775

Nature Communications (Jun 2017)

Centromeres are maintained by fastening CENP-A to DNA and directing an arginine anchor-dependent nucleosome transition

Lucie Y. Guo,
Praveen Kumar Allu,
Levani Zandarashvili,
Kara L. McKinley,
Nikolina Sekulic,
Jennine M. Dawicki-McKenna,
Daniele Fachinetti,
Glennis A. Logsdon,
Ryan M. Jamiolkowski,
Don W. Cleveland,
Iain M. Cheeseman,
Ben E. Black

Affiliations

Lucie Y. Guo: Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania
Praveen Kumar Allu: Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania
Levani Zandarashvili: Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania
Kara L. McKinley: Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology
Nikolina Sekulic: Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania
Jennine M. Dawicki-McKenna: Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania
Daniele Fachinetti: Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California, San Diego
Glennis A. Logsdon: Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania
Ryan M. Jamiolkowski: Graduate Program in Bioengineering, University of Pennsylvania
Don W. Cleveland: Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California, San Diego
Iain M. Cheeseman: Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology
Ben E. Black: Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania

DOI: https://doi.org/10.1038/ncomms15775
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 15

Abstract

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Centromere maintenance depends on the persistence of the histone variant CENP-A at the centromeres. Here, the authors characterize the core centromeric nucleosome complex wherein CENP-C confers a stable CENP-A nucleosome conformation and CENP-N fastens CENP-A to the DNA.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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