Three-dimensional characteristics of T cells and vasculature in the development of mouse esophageal cancer
Shanshan Bi,
Yueguang Wu,
Ning Ding,
Yan Zhou,
Huijuan Liu,
Yongjia Weng,
Qiqin Song,
Li Zhang,
Matthew Yibo Cheng,
Heyang Cui,
Weimin Zhang,
Yongping Cui
Affiliations
Shanshan Bi
Cancer Institute, Shenzhen Peking University-the Hong Kong University of Science and Technology Medical Center, Shenzhen 518035, P.R. China; Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen 518132, P.R. China
Yueguang Wu
Cancer Institute, Shenzhen Peking University-the Hong Kong University of Science and Technology Medical Center, Shenzhen 518035, P.R. China; Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen 518132, P.R. China
Ning Ding
Cancer Institute, Shenzhen Peking University-the Hong Kong University of Science and Technology Medical Center, Shenzhen 518035, P.R. China; Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen 518132, P.R. China
Yan Zhou
Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen 518132, P.R. China; Key Laboratory of Cellular Physiology of the Ministry of Education, Department of Pathology, Shanxi Medical University, Taiyuan 030001, P.R. China
Huijuan Liu
Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen 518132, P.R. China; Key Laboratory of Cellular Physiology of the Ministry of Education, Department of Pathology, Shanxi Medical University, Taiyuan 030001, P.R. China
Yongjia Weng
Cancer Institute, Shenzhen Peking University-the Hong Kong University of Science and Technology Medical Center, Shenzhen 518035, P.R. China; Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen 518132, P.R. China
Qiqin Song
Cancer Institute, Shenzhen Peking University-the Hong Kong University of Science and Technology Medical Center, Shenzhen 518035, P.R. China; Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen 518132, P.R. China
Li Zhang
Cancer Institute, Shenzhen Peking University-the Hong Kong University of Science and Technology Medical Center, Shenzhen 518035, P.R. China; Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen 518132, P.R. China
Matthew Yibo Cheng
Cancer Institute, Shenzhen Peking University-the Hong Kong University of Science and Technology Medical Center, Shenzhen 518035, P.R. China; Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen 518132, P.R. China
Heyang Cui
Cancer Institute, Shenzhen Peking University-the Hong Kong University of Science and Technology Medical Center, Shenzhen 518035, P.R. China; Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen 518132, P.R. China; Department of Surgery, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, SAR, China
Weimin Zhang
Cancer Institute, Shenzhen Peking University-the Hong Kong University of Science and Technology Medical Center, Shenzhen 518035, P.R. China; Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen 518132, P.R. China; Key Laboratory of Cellular Physiology of the Ministry of Education, Department of Pathology, Shanxi Medical University, Taiyuan 030001, P.R. China; State Key Laboratory of Molecular Oncology, Beijing Key Laboratory of Carcinogenesis and Translational Research, Laboratory of Molecular Oncology, Peking University Cancer Hospital & Institute, Research Unit of Molecular Cancer Research, Chinese Academy of Medical Sciences, Beijing 100142, P.R. China; Corresponding author
Yongping Cui
Cancer Institute, Shenzhen Peking University-the Hong Kong University of Science and Technology Medical Center, Shenzhen 518035, P.R. China; Institute of Cancer Research, Shenzhen Bay Laboratory, Shenzhen 518132, P.R. China; Key Laboratory of Cellular Physiology of the Ministry of Education, Department of Pathology, Shanxi Medical University, Taiyuan 030001, P.R. China; State Key Laboratory of Molecular Oncology, Beijing Key Laboratory of Carcinogenesis and Translational Research, Laboratory of Molecular Oncology, Peking University Cancer Hospital & Institute, Research Unit of Molecular Cancer Research, Chinese Academy of Medical Sciences, Beijing 100142, P.R. China; Corresponding author
Summary: Esophageal squamous cell carcinoma (ESCC) is a common malignancy, characterized by a multistep pathogenic process regulated spatiotemporally within the esophageal epithelial microenvironment, including vessel normalization and immune infiltration. However, empirical evidence elucidating esophageal vascular remodeling and immune infiltration during ESCC tumorigenesis in situ is lacking. In this study, utilizing a mouse model recapitulating progressive human ESCC stages, we established a tissue clearing workflow for three-dimensional visualization and analysis of esophageal vessels and T cell distribution. Through this workflow, we delineated the spatial dynamics of vascular remodeling, CD3+ T cells, and characteristic T cell aggregates employing high-resolution light-sheet fluorescence microscopy across five ESCC pathogenic stages. Vessel remodeling might be coupled with T cell infiltration, and their interactions predominantly occurred at the inflammatory stage. These findings provided insights into research methodologies of esophageal cancer and spatiotemporal landscapes of vascular and T cell during ESCC initiation and progression.
