Establishment and characterization of induced pluripotent stem cell (iPSCs) line UNIBSi014-A from a healthy female donor

Federica Bono; Veronica Mutti; Giovanna Piovani; Alessandra Minelli; Jessica Mingardi; Adele Guglielmi; Cristina Missale; Massimo Gennarelli; Chiara Fiorentini; Alessandro Barbon

Stem Cell Research (Mar 2021)

Establishment and characterization of induced pluripotent stem cell (iPSCs) line UNIBSi014-A from a healthy female donor

Federica Bono,
Veronica Mutti,
Giovanna Piovani,
Alessandra Minelli,
Jessica Mingardi,
Adele Guglielmi,
Cristina Missale,
Massimo Gennarelli,
Chiara Fiorentini,
Alessandro Barbon

Affiliations

Federica Bono: Division of Pharmacology, Department of, Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy
Veronica Mutti: Division of Pharmacology, Department of, Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy
Giovanna Piovani: Division of Biology and Genetics, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy
Alessandra Minelli: Division of Biology and Genetics, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy; Genetic Unit, IRCCS Istituto Centro San Giovanni di Dio Fatebenefratelli, Brescia, Italy
Jessica Mingardi: Division of Biology and Genetics, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy
Adele Guglielmi: Division of Pharmacology, Department of, Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy
Cristina Missale: Division of Pharmacology, Department of, Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy
Massimo Gennarelli: Division of Biology and Genetics, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy; Genetic Unit, IRCCS Istituto Centro San Giovanni di Dio Fatebenefratelli, Brescia, Italy
Chiara Fiorentini: Division of Pharmacology, Department of, Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy; Corresponding author.
Alessandro Barbon: Division of Biology and Genetics, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy

Journal volume & issue: Vol. 51
p. 102216

Abstract

Read online

Peripheral blood mononuclear cells (PBMCs) derived from a healthy 40-year-old female were successfully transformed into induced pluripotent stem cells (iPSCs) by using the integration-free CytoTune-iPS Sendai Reprogramming method. The resulting iPSCs line exhibits a normal karyotype, expresses stemness markers and displays the differentiation capacity into the three germ layers. This human iPSCs line can be used as healthy control in disease modelling studies.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal