A Surface Acoustic Wave Sensor with a Microfluidic Channel for Detecting C-Reactive Protein

Ming-Jer Jeng; Ying-Chang Li; Mukta Sharma; Chia-Wei Chen; Chia-Lung Tsai; Yen-Heng Lin; Shiang-Fu Huang; Liann-Be Chang; Chao-Sung Lai

doi:10.3390/chemosensors9050106

Chemosensors (May 2021)

A Surface Acoustic Wave Sensor with a Microfluidic Channel for Detecting C-Reactive Protein

Ming-Jer Jeng,
Ying-Chang Li,
Mukta Sharma,
Chia-Wei Chen,
Chia-Lung Tsai,
Yen-Heng Lin,
Shiang-Fu Huang,
Liann-Be Chang,
Chao-Sung Lai

Affiliations

Ming-Jer Jeng: Department of Electronic Engineering, Chang Gung University, Taoyuan 333, Taiwan
Ying-Chang Li: Green Technology Research Center, Chang Gung University, Taoyuan 333, Taiwan
Mukta Sharma: Department of Electronic Engineering, Chang Gung University, Taoyuan 333, Taiwan
Chia-Wei Chen: Department of Electronic Engineering, Chang Gung University, Taoyuan 333, Taiwan
Chia-Lung Tsai: Department of Electronic Engineering, Chang Gung University, Taoyuan 333, Taiwan
Yen-Heng Lin: Department of Electronic Engineering, Chang Gung University, Taoyuan 333, Taiwan
Shiang-Fu Huang: Department of Otolaryngology-Head and Neck Surgery, Chang Gung Memorial Hospital, Linkou 244, Taiwan
Liann-Be Chang: Department of Electronic Engineering, Chang Gung University, Taoyuan 333, Taiwan
Chao-Sung Lai: Department of Electronic Engineering, Chang Gung University, Taoyuan 333, Taiwan

DOI: https://doi.org/10.3390/chemosensors9050106
Journal volume & issue: Vol. 9, no. 5
p. 106

Abstract

Read online

A surface acoustic wave (SAW) sensor with a microfluidic channel was studied to detect C-reactive protein (CRP). A piezoelectric lithium niobate substrate was used to examine the frequency response of the microfluidic SAW sensor. The amplitude (insertion loss) changes in the microfluidic SAW sensor were measured from the interaction of CRP/anti-CRP owing to mass variation. The fabricated microfluidic SAW sensor exhibited a detection limit of 4 ng/mL CRP concentration. A wide CRP concentration range (10 ng/mL to 0.1 mg/mL) can be detected by this sensor, which is higher than the existing CRP detection methods. A good linear relationship between the amplitude peak shift and CRP concentrations from 10 ng/mL to 0.1 mg/mL was obtained. The amplitude peak shifts in the sensor can be useful for estimating CRP concentration. This can be used as a biosensor to diagnose the risk of cardiovascular disease.

Published in Chemosensors

ISSN: 2227-9040 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry
Website: http://www.mdpi.com/journal/chemosensors

About the journal

Abstract

Keywords