Biomimetic Coating on Porous Alumina for Tissue Engineering: Characterisation by Cell Culture and Confocal Microscopy

Materials. 2015;8(6):3584-3606 DOI 10.3390/ma8063584

 

Journal Homepage

Journal Title: Materials

ISSN: 1996-1944 (Print)

Publisher: MDPI AG

LCC Subject Category: Technology: Electrical engineering. Electronics. Nuclear engineering | Technology: Engineering (General). Civil engineering (General) | Science: Natural history (General): Microscopy | Science: Physics: Descriptive and experimental mechanics

Country of publisher: Switzerland

Language of fulltext: English

Full-text formats available: PDF, HTML

 

AUTHORS

Elizabeth Kolos (Biomedical Engineering, AMME J07, University of Sydney, Sydney, NSW 2006, Australia)
Andrew J Ruys (Biomedical Engineering, AMME J07, University of Sydney, Sydney, NSW 2006, Australia)

EDITORIAL INFORMATION

Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 11 weeks

 

Abstract | Full Text

In this study porous alumina samples were prepared and then coated using the biomimetic coating technique using a five times Simulated Body Fluid (5.0SBF) as the growth solution. A coating was achieved after pre-treatment with concentrated acid. From elemental analysis, the coating contained calcium and phosphorous, but also sodium and chlorine. Halite was identified by XRD, a sodium chloride phase. Sintering was done to remove the halite phase. Once halite was burnt off, the calcium phosphate crystals were not covered with halite and, therefore, the apatite phases can be clearly observed. Cell culturing showed sufficient cell attachment to the less porous alumina, Sample B, that has more calcium phosphate growth, while the porous alumina, Sample A, with minimal calcium phosphate growth attained very little cell attachment. This is likely due to the contribution that calcium phosphate plays in the attachment of bone-like cells to a bioinert ceramic such as alumina. These results were repeated on both SEM and confocal microscopy analysis. Confocal microscopy was a novel characterisation approach which gave useful information and was a visual aid.