Nature Communications (Oct 2021)

Circa-SCOPE: high-throughput live single-cell imaging method for analysis of circadian clock resetting

  • Gal Manella,
  • Dan Aizik,
  • Rona Aviram,
  • Marina Golik,
  • Gad Asher

DOI
https://doi.org/10.1038/s41467-021-26210-1
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 14

Abstract

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Phase Transition Curves (PTCs) describe phase shifts of circadian oscillations due to a stimulus and they are important for studying circadian clock resetting. Here, the authors present a method for high-throughput reconstruction of PTCs using fluorescent live imaging and single-cell analysis.