Single Nucleotide Polymorphism Markers Associated with Seed Quality Characteristics of Cultivated Lentil

Abstract

The dimensions of lentil ( Medik.) seeds are important quality parameters that are major determinants of market preference, cooking time, and post-harvest milling quality. Knowledge of the genetic control of traits related to seed dimensions would be useful for crop improvement. The principal aim of this study was to identify single nucleotide polymorphism (SNP) markers linked to genes that control seed diameter, seed thickness, and seed plumpness. Association mapping analysis with SNP markers was used to study the seed dimensions of 138 diverse cultivated lentil accessions grown at two locations in Saskatchewan, Canada, in 2011 and 2012. Six marker–trait associations were shown to be significant for the studied seed dimension characteristics. Two SNP markers closely associated with seed diameter across locations and years identified in previous work were validated in this study. Three additional marker–seed thickness associations were identified. Using the association mapping strategy, we confirmed the presence of two genomic regions controlling seed diameter and plumpness. This information can be used worldwide as a resource for lentil seed quality improvement programs.