Transcriptome analysis of strawberry (Fragaria × ananasa) responsive to Colletotrichum gloeosporioides inoculation and mining of resistance genes

Wenwei Liang; Mingjie Wang; Binghao Du; Lei Ling; Yingdong Bi; Jinghua Zhang; Yimin Sun; Shuang Zhou; Lili Zhang; Xiao Ma; Jun Ma; Liren Wu; Changhong Guo

doi:10.1080/13102818.2022.2106886

Biotechnology & Biotechnological Equipment (Dec 2022)

Transcriptome analysis of strawberry (Fragaria × ananasa) responsive to Colletotrichum gloeosporioides inoculation and mining of resistance genes

Wenwei Liang,
Mingjie Wang,
Binghao Du,
Lei Ling,
Yingdong Bi,
Jinghua Zhang,
Yimin Sun,
Shuang Zhou,
Lili Zhang,
Xiao Ma,
Jun Ma,
Liren Wu,
Changhong Guo

Affiliations

Wenwei Liang: Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin, Heilongjiang, PR China
Mingjie Wang: Grape Laboratory, Gardening Branch, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang, PR China
Binghao Du: Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin, Heilongjiang, PR China
Lei Ling: Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin, Heilongjiang, PR China
Yingdong Bi: Soybean Laboratory, Crop Tillage and Cultivation Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang, PR China
Jinghua Zhang: Berry Resources Laboratory, Institute of Rural Revitalization Science and Technology, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang, PR China
Yimin Sun: Berry Resources Laboratory, Institute of Rural Revitalization Science and Technology, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang, PR China
Shuang Zhou: Berry Resources Laboratory, Institute of Rural Revitalization Science and Technology, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang, PR China
Lili Zhang: Berry Resources Laboratory, Institute of Rural Revitalization Science and Technology, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang, PR China
Xiao Ma: Berry Resources Laboratory, Institute of Rural Revitalization Science and Technology, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang, PR China
Jun Ma: Resources Laboratory, Cash Crop Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang, PR China
Liren Wu: Resources Laboratory, Cash Crop Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang, PR China
Changhong Guo: Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin, Heilongjiang, PR China

DOI: https://doi.org/10.1080/13102818.2022.2106886
Journal volume & issue: Vol. 36, no. 1
pp. 684 – 696

Abstract

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AbstractStrawberry has high nutritional and economic value and is an important horticultural crop widely cultivated worldwide. Strawberry anthracnose is the most harmful disease in strawberry production, which causes huge production losses every year. Mining disease resistance genes and breeding high-quality strawberry varieties are the fundamental way to solve the problem of strawberry anthracnose. In this study, transcriptome analysis and disease resistance-related genes mining were targeted on strawberry anthracnose. The main results are as follows: (1) A total of 100.45 Gb clean reads were obtained, with no less than 6.26 Gb for each sample; (2) The clean reads for each sample were aligned to the reference genome efficiently at 91.30% − 92.27%; (3) 93,042 genes were annotated; (4) 16 anti-anthracnose-related genes were identified, including FaAUX1, FaARF18, FaSAUR50, FaGH3.6, FaAHP1, FaARR (3, 5 and 11), FaPYR1, FaPYL11, FaPP2C (6, 16, 24, 37 and 51) and FaPR1. The above results can provide theoretical guidance for the molecular breeding of disease resistance of strawberry.

Published in Biotechnology & Biotechnological Equipment

ISSN: 1310-2818 (Print); 1314-3530 (Online)
Publisher: Taylor & Francis Group
Country of publisher: United Kingdom
LCC subjects: Technology: Chemical technology: Biotechnology
Website: https://www.tandfonline.com/journals/tbeq

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