Adulticidal activity and chemical composition of the essential oil of Lantana camara leaves in Drosophila melanogaster | [Actividad adulticida y composición química del aceite esencial de hojas de Lantana camara sobre Drosophila melanogaster[

Abstract

The indiscriminate use of synthetic pesticides in the control of pests and diseases affects crops, human health, and the environment, and lead to insect resistance and the presence of toxic residues in food. Previous generated the need to develop alternative approaches to pest species control. In the present study the insecticidal activity of the essential oil of leaves of Lantana camara on the insect model Drosophila melanogaster has been investigated. Essential oil was isolated from L. camara leaves using the hydro-distillation method. The Bioassay test was performed by the WHO method for the determination of adulticidal activity against mosquitoes and arthropods. Different compounds were identified by gas chromatography - mass spectrometry analysis. The LC50 value of the oil was 0.56 mg cm-2 whereas the value of the LC95 was 0.96 mg cm-2 on Drosophila melanogaster. The yield of leaf essential oil obtained by hydro-distillation was 0.022% w/w. Gas chromatography-mass spectrometry analysis of the essential oil showed 66 peaks, where Germacren D (19.29%), B-Caryophyllene (14.55%), α-Humulene (9.51%), Biciclogermacren (8.94%), Germacren B (7.26%) and γ-Terpinene (5.62%) were present in greater amounts and contributed 56.21% of the total constituents. The essential oil of L. camara leaves has adulticidal activity on the fly species Drosophila melanogaster and could be used for the development of oil-based insecticides as a complement to synthetic insecticides for the control of flies causing important economic losses.

Keywords

• L. Camara
• essential oil
• CG-MS
• CL50-CL95
• bioassay
• insecticide