Orchestration of Processing Body Dynamics and mRNA Decay in Arabidopsis Immunity
Xiao Yu,
Bo Li,
Geng-Jen Jang,
Shan Jiang,
Daohong Jiang,
Jyan-Chyun Jang,
Shu-Hsing Wu,
Libo Shan,
Ping He
Affiliations
Xiao Yu
Department of Plant Pathology and Microbiology and Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, TX 77843, USA
Bo Li
Department of Plant Pathology and Microbiology and Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, TX 77843, USA; Department of Biochemistry and Biophysics and Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, TX 77843, USA; Provincial Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, P.R. China
Geng-Jen Jang
Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan
Shan Jiang
Department of Plant Pathology and Microbiology and Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, TX 77843, USA
Daohong Jiang
Provincial Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, P.R. China
Jyan-Chyun Jang
Department of Horticulture and Crop Science, Department of Molecular Genetics, Center for Applied Plant Sciences, Center for RNA Biology, Ohio State University, Columbus, OH 43210, USA
Shu-Hsing Wu
Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan
Libo Shan
Department of Plant Pathology and Microbiology and Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, TX 77843, USA
Ping He
Department of Biochemistry and Biophysics and Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, TX 77843, USA; Corresponding author
Summary: Proper transcriptome reprogramming is critical for hosts to launch an effective defense response upon pathogen attack. How immune-related genes are regulated at the posttranscriptional level remains elusive. We demonstrate here that P-bodies, the non-membranous cytoplasmic ribonucleoprotein foci related to 5′-to-3′ mRNA decay, are dynamically modulated in plant immunity triggered by microbe-associated molecular patterns (MAMPs). The DCP1-DCP2 mRNA decapping complex, a hallmark of P-bodies, positively regulates plant MAMP-triggered responses and immunity against pathogenic bacteria. MAMP-activated MAP kinases directly phosphorylate DCP1 at the serine237 residue, which further stimulates its interaction with XRN4, an exonuclease executing 5′-to-3′ degradation of decapped mRNA. Consequently, MAMP treatment potentiates DCP1-dependent mRNA decay on a specific group of MAMP-downregulated genes. Thus, the conserved 5′-to-3′ mRNA decay elicited by the MAMP-activated MAP kinase cascade is an integral part of plant immunity. This mechanism ensures a rapid posttranscriptional downregulation of certain immune-related genes that may otherwise negatively impact immunity. : Yu et al. show that P-body core components DCP1 and DCP2 positively regulate plant pattern-triggered immunity. DCP1 phosphorylation by immune-activated MAPKs contributes to P-body disassembly and mRNA decay on a subset of immune-regulated genes, revealing mRNA-decay-mediated posttranscriptional regulation is an integral part of plant immunity. Keywords: plant immunity, microbe-associated molecular patterns, processing body, mRNA decay, mRNA decapping complex, MAP kinases
