Employing nanobodies for immune landscape profiling by PET imaging in mice

Abraham J.P. Teunissen; Omar B. Abousaway; Jazz Munitz; Mandy M.T. van Leent; Yohana C. Toner; Bram Priem; Max L. Senders; Carlos Pérez-Medina; Willem J.M. Mulder; Mohammad Rashidian

STAR Protocols (Jun 2021)

Employing nanobodies for immune landscape profiling by PET imaging in mice

Abraham J.P. Teunissen,
Omar B. Abousaway,
Jazz Munitz,
Mandy M.T. van Leent,
Yohana C. Toner,
Bram Priem,
Max L. Senders,
Carlos Pérez-Medina,
Willem J.M. Mulder,
Mohammad Rashidian

Affiliations

Abraham J.P. Teunissen: Biomedical Engineering and Imaging Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Corresponding author
Omar B. Abousaway: Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02215-5418, USA
Jazz Munitz: Biomedical Engineering and Imaging Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA
Mandy M.T. van Leent: Biomedical Engineering and Imaging Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA
Yohana C. Toner: Biomedical Engineering and Imaging Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA
Bram Priem: Biomedical Engineering and Imaging Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Department of Medical Biochemistry, Amsterdam UMC, Amsterdam 1105 AZ, the Netherlands; Angiogenesis Laboratory, Amsterdam UMC, Cancer Center Amsterdam, Amsterdam 1105 AZ, the Netherlands
Max L. Senders: Biomedical Engineering and Imaging Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA
Carlos Pérez-Medina: Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid 28029, Spain
Willem J.M. Mulder: Biomedical Engineering and Imaging Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Department of Pharmacological Sciences and Mount Sinai Center for Therapeutics Discovery, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA
Mohammad Rashidian: Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02215-5418, USA; Corresponding author

Journal volume & issue: Vol. 2, no. 2
p. 100434

Abstract

Read online

Summary: Noninvasive immunoimaging holds great potential for studying and stratifying disease as well as therapeutic efficacy. Radiolabeled single-domain antibody fragments (i.e., nanobodies) are appealing probes for immune landscape profiling, as they display high stability, rapid targeting, and excellent specificity, while allowing extremely sensitive nuclear readouts. Here, we present a protocol for radiolabeling an anti-CD11b nanobody and studying its uptake in mice by a combination of positron emission tomography imaging, ex vivo gamma counting, and autoradiography. Our protocol is applicable to nanobodies against other antigens.For complete details on the use and execution of this protocol, please see Priem et al. (2020), Senders et al. (2019), or Rashidian et al. (2017).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

About the journal

Abstract

Keywords