Acta Cirúrgica Brasileira (Jan 2020)

Effect of norbixin-based poly(hydroxybutyrate) membranes on the tendon repair process after tenotomy in rats

  • Lízia Daniela e Silva Nascimento,
  • Renata Amadei Nicolau,
  • Antônio Luiz Martins Maia Filho,
  • José Zilton Lima Verde Santos,
  • Khetyma Moreira Fonseca,
  • Danniel Cabral Leão Ferreira,
  • Rayssilane Cardoso de Sousa,
  • Vicente Galber Freitas Viana,
  • Luiz Fernando Meneses Carvalho,
  • José Figueredo-Silva

DOI
https://doi.org/10.1590/s0102-865020190110000001
Journal volume & issue
Vol. 34, no. 11

Abstract

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Abstract Purpose: To determine the efficacy of norbixin-based poly(hydroxybutyrate) (PHB) membranes for Achilles tendon repair. Methods: Thirty rats were submitted to total tenotomy surgery of the right Achilles tendon and divided into two groups (control and membrane; n = 15 each), which were further subdivided into three subgroups (days 7, 14, and 21; n = 5 each). Samples were analyzed histologically. Results: Histological analysis showed a significant reduction in inflammatory infiltrates on days 7, 14 (p < 0.0001 for both), and 21 (p = 0.0004) in the membrane group compared to that in the control group. There was also a significant decrease in the number of fibroblasts in the control group on days 7, 14 (p < 0.0001), and 21 (p = 0.0032). Further, an increase in type I collagen deposition was observed in the membrane group compared to that in the control group on days 7 (p = 0.0133) and 14 (p = 0.0107). Conclusion: Treatment with norbixin-based PHB membranes reduces the inflammatory response, increases fibroblast proliferation, and improves collagen production in the tendon repair region, especially between days 7 and 14.

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