Activity of ligninolytic enzyme of Pleurotus ostreatus on media containing OPEFB and their application for dyes decolorization

Abstract

Ligninolytic enzymes are known as extracellular enzymes produced by the white rot fungi class of basidiomycetes. One of the most well-known fungi of the white rot fungus is Pleurotus ostreatus. The aim of this study to calculate the activity of ligninolytic enzymes in the growth media of Pleurotus ostreatus and their application in decolorization of dye colour. The ligninolytic enzyme extract obtained was used to decolorize blue dyes (Methylene Blue) and red dyes (Congo Red). The highest laccase enzyme activity was in the first month of 0.35 U/mL with E1 media composition; the highest manganese peroxidase (MnP) enzyme activity was in the fourth month at 31.818 U / mL with E4 media composition; and the highest lignin peroxidase (LiP) enzyme activity was in the fifth month at 0.269 U / mL with E1 media composition. The enzyme extract obtained was then applied to decolorize red and blue dyes. Decolorization of dyes was measured using spectrophotometry with a blue wavelength of 470 nm and red 685 nm. The highest reduction in decolorization of blue dye and red dye was 12 hours with concentration of enzyme addition of 0.5%. Based on these results, ligninolytic enzymes potentially to be developed as bioactive agents for detergents.

Keywords

• decolorization
• laccase
• mangan peroxidase
• lignin peroxidase
• spectrofotometry