Evaluation of Immunobiological Properties of Bordetella pertussis Vaccine Strains

Abstract

The study performed as part of evaluation of immunobiological properties of Bordetella pertussis vaccine strains offered an opportunity to standardize pertussis bacterial mass based on the content of surface antigens — agglutinogens 1, 2 and 3. Certain levels of agglutinogens in seed lots were found to be capable of modifying the protective activity of pertussis vaccine. The heterogeneity of the bacterial strain population was reduced by applying selection criteria for colonies based on expression of agglutinogens. B. pertussis seed lots derived from vaccine strains that had undergone selection were sent to manufacturers of the pertussis component of DTP vaccine. Production of pertussis vaccine using a more homogenous bacterial mass that actively expresses agglutinogens 1, 2 and 3 made it possible to enhance immunogenic activity of the pertussis component of DTP vaccine. The safety of the whole-cell pertussis vaccine produced from the standardized seed lot was not compromised.

Keywords

• whole-cell pertussis vaccine
• pertussis component of dtp vaccine
• agglutinogens
• selection of bordetella pertussis colonies
• protective activity of pertussis vaccine