<em>Escherichia coli</em> Strains Responsible for Cystitis in Female Pediatric Patients with Normal and Abnormal Urinary Tracts Have Different Virulence Profiles
Marta de Oliveira Domingos,
Silvio Marciano da Silva Junior,
Wagner Milanello,
Shirley Sizue Nakamura Nakano,
Marcia Regina Franzolin,
Luis Fernando dos Santos,
Kamila Oliveira Nunes,
Vaniky Duarte Marques,
Waldir P. Elias,
Herbert Guimarães de Sousa Silva,
Bruna De Lucca Caetano,
Roxane Maria Fontes Piazza
Affiliations
Marta de Oliveira Domingos
Laboratório de Bacteriologia, Instituto Butantan, Avenida Vital Brasil, 1500, São Paulo 05503-900, SP, Brazil
Silvio Marciano da Silva Junior
Laboratório de Bacteriologia, Instituto Butantan, Avenida Vital Brasil, 1500, São Paulo 05503-900, SP, Brazil
Wagner Milanello
Hospital Infantil Darcy Vargas, Rua Seráfico de Assis Carvalho, 34, São Paulo 05614-040, SP, Brazil
Shirley Sizue Nakamura Nakano
Hospital Infantil Darcy Vargas, Rua Seráfico de Assis Carvalho, 34, São Paulo 05614-040, SP, Brazil
Marcia Regina Franzolin
Laboratório de Bacteriologia, Instituto Butantan, Avenida Vital Brasil, 1500, São Paulo 05503-900, SP, Brazil
Luis Fernando dos Santos
Centro de Bacteriologia, Núcleo de Doenças Entéricas, Instituto Adolfo Lutz, Avenida Dr. Arnaldo, 355, São Paulo 01246-000, SP, Brazil
Kamila Oliveira Nunes
Laboratório de Bacteriologia, Instituto Butantan, Avenida Vital Brasil, 1500, São Paulo 05503-900, SP, Brazil
Vaniky Duarte Marques
Laboratório de Bacteriologia, Instituto Butantan, Avenida Vital Brasil, 1500, São Paulo 05503-900, SP, Brazil
Waldir P. Elias
Laboratório de Bacteriologia, Instituto Butantan, Avenida Vital Brasil, 1500, São Paulo 05503-900, SP, Brazil
Herbert Guimarães de Sousa Silva
Laboratório de Bacteriologia, Instituto Butantan, Avenida Vital Brasil, 1500, São Paulo 05503-900, SP, Brazil
Bruna De Lucca Caetano
Laboratório de Bacteriologia, Instituto Butantan, Avenida Vital Brasil, 1500, São Paulo 05503-900, SP, Brazil
Roxane Maria Fontes Piazza
Laboratório de Bacteriologia, Instituto Butantan, Avenida Vital Brasil, 1500, São Paulo 05503-900, SP, Brazil
The role of uropathogenic Escherichia coli (UPEC) in colonization and infection of female patients with anatomical and functional abnormalities of the urinary system is elusive. In this study, the phenotype, genotype and the phylogeny of UPEC strains isolated from the urine of pediatric female patients with cystitis of normal and abnormal urinary tract were determined. Multiplex PCR results demonstrated that 86% of the strains isolated from female patients with normal urinary tract (NUT), belonged to the phylo-groups B2 and D. Their prevalence decreased to 23% in strains isolated from patients with abnormal urinary tract (AUT). More of the isolates from AUT patients produced a biofilm on polystyrene and polyvinyl chloride (PVC), adhered to epithelial cells, and encoded pap and sfa genes than strains isolated from female patients with NUT. In contrast, a higher number of hemolysin-producing strains with serogroups associated with UPEC were isolated from patients with NUT. In summary, the results suggest that cystitis in female patients with NUT is associated with ExPEC, whereas cystitis in female patients with AUT is associated with pathogenic intestinal E. coli strains that have acquired the ability to colonize the bladder.
