Cloning and Characterization of Protein Prenyltransferase Alpha Subunit in Rice
Wang Tao,
Lou Lijuan,
Li Zeyu,
Shang Lianguang,
Wang Quan
Affiliations
Wang Tao
Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture/Genome Analysis Laboratory of Ministry of Agriculture and Rural Affairs/Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
Lou Lijuan
Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture/Genome Analysis Laboratory of Ministry of Agriculture and Rural Affairs/Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
Li Zeyu
Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture/Genome Analysis Laboratory of Ministry of Agriculture and Rural Affairs/Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
Shang Lianguang
Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture/Genome Analysis Laboratory of Ministry of Agriculture and Rural Affairs/Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
Wang Quan
Corresponding author.; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture/Genome Analysis Laboratory of Ministry of Agriculture and Rural Affairs/Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
Protein prenylation plays a crucial role in plant development and stress response. We report the function of prenyltransferase α-subunit in rice. Protein-protein interactions showed that the farnesyl- transferase (OsPFT)/geranylgeranyltransferase-I (OsPGGT I-α) protein interacted together with OsPFT-β and OsPGGT I-β. The α- and β-subunits of OsPFT formed a heterodimer for the transfer of a farnesyl group from farnesyl pyrophosphate to the CaaX-box-containing peptide N-dansyl-GCVLS. Furthermore, the tissue expression patterns of the OsPFT and OsPGGT I subunits were similar, and these subunits were localized in the cytoplasm and nucleus. Moreover, OsPFT/OsPGGT I-α-deletion homozygous rice mutants had a lethal phenotype, and the heterozygous mutants exhibited reduced pollen viability. These results indicated that prenylation plays an important role in rice development.
