Quantification of total apolipoprotein E and its specific isoforms in cerebrospinal fluid and blood in Alzheimer’s disease and other neurodegenerative diseases

Melinda Rezeli; Henrik Zetterberg; Kaj Blennow; Ann Brinkmalm; Thomas Laurell; Oskar Hansson; György Marko-Varga

doi:10.1016/j.euprot.2015.07.012

EuPA Open Proteomics (Sep 2015)

Quantification of total apolipoprotein E and its specific isoforms in cerebrospinal fluid and blood in Alzheimer’s disease and other neurodegenerative diseases

Melinda Rezeli,
Henrik Zetterberg,
Kaj Blennow,
Ann Brinkmalm,
Thomas Laurell,
Oskar Hansson,
György Marko-Varga

Affiliations

Melinda Rezeli: Division of Clinical Protein Science & Imaging, Department of Biomedical Engineering, Lund University, Lund, Sweden
Henrik Zetterberg: Clinical Neurochemistry Laboratory, Institute of Neuroscience and Physiology, The Sahlgrenska Academy at University of Gothenburg, Mölndal, Sweden
Kaj Blennow: Clinical Neurochemistry Laboratory, Institute of Neuroscience and Physiology, The Sahlgrenska Academy at University of Gothenburg, Mölndal, Sweden
Ann Brinkmalm: Clinical Neurochemistry Laboratory, Institute of Neuroscience and Physiology, The Sahlgrenska Academy at University of Gothenburg, Mölndal, Sweden
Thomas Laurell: Division of Clinical Protein Science & Imaging, Department of Biomedical Engineering, Lund University, Lund, Sweden
Oskar Hansson: Clinical Memory Research Unit, Department of Clinical Sciences, Lund University, Malmö, Sweden
György Marko-Varga: Division of Clinical Protein Science & Imaging, Department of Biomedical Engineering, Lund University, Lund, Sweden

DOI: https://doi.org/10.1016/j.euprot.2015.07.012
Journal volume & issue: Vol. 8, no. C
pp. 137 – 143

Abstract

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A targeted mass spectrometric assay was developed for identification and quantification of apoE isoforms (apoE2, E3 and E4), and it was utilized for screening of samples from AD patients (n = 39) and patients with other neurodegenerative disorders (n = 38). The assay showed good linearity with LOQ corresponds to total apoE concentration of 0.8 and 40 ng/mL in CSF and plasma/serum, respectively. We identified apoE phenotypes with 100% accuracy in clinical samples. We found strong association between genotypes of the individuals and their apoE levels in blood; ϵ4 allele carriers had significantly lower apoE levels in blood than non-carriers.

Published in EuPA Open Proteomics

ISSN: 2212-9685 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General): Genetics
Website: http://www.journals.elsevier.com/eupa-open-proteomics/

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