Generation of an induced pluripotent stem cell line from a type 1 neurofibromatosis patient with NF1 mutation

Hongmei Xin; Yixiao Li; Kaihui Zhang; Aihua Ji; Meili Fan

Stem Cell Research (Dec 2024)

Generation of an induced pluripotent stem cell line from a type 1 neurofibromatosis patient with NF1 mutation

Hongmei Xin,
Yixiao Li,
Kaihui Zhang,
Aihua Ji,
Meili Fan

Affiliations

Hongmei Xin: Pediatric Research Institute, Children’s Hospital Affiliated to Shandong University, Jinan 250022, China
Yixiao Li: Pediatric Research Institute, Children’s Hospital Affiliated to Shandong University, Jinan 250022, China; Department of Ophthalmology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250001, China
Kaihui Zhang: Pediatric Research Institute, Children’s Hospital Affiliated to Shandong University, Jinan 250022, China
Aihua Ji: Department of Dermatology, Children’s Hospital Affiliated to Shandong University (Jinan Children’s Hospital), Jinan, Shandong 250022, China; Corresponding authors.
Meili Fan: Tuina Department, Children’s Hospital Affiliated to Shandong University (Jinan Children’s Hospital), Jinan, Shandong 250022, China; Corresponding authors.

Journal volume & issue: Vol. 81
p. 103568

Abstract

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A human induced pluripotent stem cell (iPSC) line was generated from patient with type 1 neurofibromatosis (NF-1), carrying heterozygous mutation in NF1 gene. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non-integrating delivery of KFL4, OCT4, SOX2, BCL-XL and c-MYC. The iPSC line expresses pluripotency markers, displays a normal karyotype, and is able to differentiate into three germ layers in vitro. This iPSC line represents a valuable cell model for NF1 in humans.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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