A Gustatory Receptor GR8 Tunes Specifically to D-Fructose in the Common Cutworm <i>Spodoptera litura</i>
Xiao-Long Liu,
Qi Yan,
Yi-Lin Yang,
Wen Hou,
Chun-Li Miao,
Ying-Chuan Peng,
Shuang-Lin Dong
Affiliations
Xiao-Long Liu
Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/Department of Entomology, Nanjing Agricultural University, Nanjing 210095, China
Qi Yan
Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/Department of Entomology, Nanjing Agricultural University, Nanjing 210095, China
Yi-Lin Yang
Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/Department of Entomology, Nanjing Agricultural University, Nanjing 210095, China
Wen Hou
Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/Department of Entomology, Nanjing Agricultural University, Nanjing 210095, China
Chun-Li Miao
Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/Department of Entomology, Nanjing Agricultural University, Nanjing 210095, China
Ying-Chuan Peng
Institute of Entomology, Jiangxi Agricultural University, Nanchang 330045, China
Shuang-Lin Dong
Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/Department of Entomology, Nanjing Agricultural University, Nanjing 210095, China
Gustatory receptors (GRs) are crucial in the peripheral coding of the non-volatile compounds in insects, and thus play important roles in multiple behaviors including feeding, mating, and oviposition. However, little research has been done on GRs in lepidopteran pests. In the current work with Spodoptera litura, an important worldwide crop’s pest, a candidate fructose GR gene (SlitGR8) was cloned in full length, and its spatial and temporal expression profiles were determined by quantitative real-time PCR (qPCR). It revealed that SlitGR8 was highly expressed in antennae of both male and female adults, as well as in larva of first, fifth and sixth instar. Functional analyses were further conducted using the Xenopus oocyte system. SlitGR8 responded specifically to D-fructose among 12 tested sugar compounds. In addition, the behavioral assay demonstrated that both female and male moths could respond with proboscis extension behavior to D-fructose applied onto the antenna, but females showed higher sensitivity than males. The results provide an important base for further elucidation of molecular mechanisms of gustation, and a potential target for development of feeding interfering technique in S. litura.