The electrospinning electric field is the main factor responsible for the jet stretching force. Previous studies showed that nonhomogeneous electric field would affect the jets formed during the multi-needles electrospinning. Therefore, a multitude of methods were explored to optimize the electric field in multi-needles electrospinning, such as adjusting the supplied voltage on each needle, varying the space of the needles and spatial distribution of the needles. In this paper, three categories of needles distribution were explored for a homogeneous electric field and the design of a higher needles density of electrospinning head. The theoretical and experimental results showed that the homogeneous electric field formed with the trapezoidal head at 15mm distance while the others’ head required over 30mm. Hence, the trapezoidal arrangement is a better multi-needles distribution for electrospinning head, and has much more significance for high yield of electrospinning nanofibers.