Diagnostic Pathology (Sep 2008)

The relative test performance characteristics of two commercial assays for the detection of <it>Mycobacterium tuberculosis </it>complex in paraffin-fixed human biopsy specimens

  • Broukhanski George,
  • Lombos Ernesto,
  • Chedore Pam,
  • Pyskir Daria,
  • Eshaghi AliReza,
  • Drews Steven J,
  • Higgins Rachel,
  • Fisman David N,
  • Blair Joanne,
  • Jamieson Frances

DOI
https://doi.org/10.1186/1746-1596-3-37
Journal volume & issue
Vol. 3, no. 1
p. 37

Abstract

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Abstract The Seeplex™ TB Detection-2 assay (Rockville, MD) is a nested endpoint PCR for the Mycobacterium tuberculosis complex (MTBC) targets IS6110 and MPB64 that utilizes dual priming oligonucleotide technology. When used to detect the presence of MTBC DNA in formalin-fixed paraffin-embedded tissue specimens, the sensitivity and specificity of this assay is equivalent to a labor-intensive traditional endpoint PCR assay and is more sensitive than a commercial real-time PCR assay.