In vitro regeneration and Agrobacterium-mediated genetic transformation of Caragana korshinskii

Bin Liu; Xiaorui Shang; Xuting Zhang; Wen Shao; Lulu Ren; Guojing Li; Mulan Zhu; Ruigang Wang

doi:10.48130/FR-2023-0014

Forestry Research (Jan 2023)

In vitro regeneration and Agrobacterium-mediated genetic transformation of Caragana korshinskii

Bin Liu,
Xiaorui Shang,
Xuting Zhang,
Wen Shao,
Lulu Ren,
Guojing Li,
Mulan Zhu,
Ruigang Wang

Affiliations

Bin Liu: Key Laboratory of Plants Adversity Adaptation and Genetic Improvement in Cold and Arid Regions of Inner Mongolia, Inner Mongolia Agricultural University, Hohhot 010018, China
Xiaorui Shang: Key Laboratory of Plants Adversity Adaptation and Genetic Improvement in Cold and Arid Regions of Inner Mongolia, Inner Mongolia Agricultural University, Hohhot 010018, China
Xuting Zhang: Key Laboratory of Plants Adversity Adaptation and Genetic Improvement in Cold and Arid Regions of Inner Mongolia, Inner Mongolia Agricultural University, Hohhot 010018, China
Wen Shao: Shanghai Key Laboratory of Plant Functional Genomics and Resources , Shanghai Chenshan Botanical Garden, Shanghai 201602, China
Lulu Ren: State Key Laboratory of Tea Plant Biology and Utilization/ Key Laboratory of Tea Biology and Tea Processing, Anhui Agricultural University, Hefei 230036, China
Guojing Li: Key Laboratory of Plants Adversity Adaptation and Genetic Improvement in Cold and Arid Regions of Inner Mongolia, Inner Mongolia Agricultural University, Hohhot 010018, China
Mulan Zhu: CAS Center for Excellence in Molecular Plant Science, Shanghai Chenshan Plant Science Research Center, Chinese Academy of Science, Shanghai 201602, China
Ruigang Wang: Key Laboratory of Plants Adversity Adaptation and Genetic Improvement in Cold and Arid Regions of Inner Mongolia, Inner Mongolia Agricultural University, Hohhot 010018, China

DOI: https://doi.org/10.48130/FR-2023-0014
Journal volume & issue: Vol. 3, no. 1
pp. 1 – 10

Abstract

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Caragana korshinskii is a deciduous shrub with large eco-economic value and strong tolerance to abiotic stresses. However, the shortage of reliable genetic transformation technology severely hinders its research on stress tolerance mechanisms and stress-resistant gene mining and application. In this study, the embryonic tip of the C. korshinskii seedling was used as the initiating explant to get regenerated plant through the direct organogenesis pathway, which significantly shortened the culture cycle and set the foundation for investigation of Agrobacterium-mediated genetic transformation. Our results suggest that the embryonic tip possesses robust meristem capacity and is an efficient method for transgenic breeding. This research provides a technical basis for asexual reproduction, molecular breeding, and gene function investigation in C. korshinskii by establishing, for the first time, an effective in vitro regeneration system and an Agrobacterium-mediated stable genetic transformation system utilizing the embryonic tip of C. korshinskii as explants.

Published in Forestry Research

ISSN: 2767-3812 (Online)
Publisher: Maximum Academic Press
Country of publisher: China
LCC subjects: Agriculture: Forestry
Website: https://www.maxapress.com/forres

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