Evaluation of CD30 expression in B ALL and its correlation with MRD(Minimum Residual Disease)

Abstract

Background: This study was conducted to evaluate CD30 expression in minimum residual disease after chemotherapy in B-acute lymphoblastic leukemia (B-ALL). Materials and Methods: This was a cross-sectional study on 30 new cases of B-ALL between 2018 and 2019. We checked CD30 expressions in fresh bone marrow aspirates by flow cytometry. After 28 days of routine chemotherapy, we calculated minimal residual disease in CD30 positive and negative patients and compare them by Kolmogorov–Smirnov test. Results: Thirty patients with B-ALL with a mean age of 15.62 ± 20.488 were included in the study. CD30 marker was positive in about 10 patients and was negative in about 20 participants. Mean blast count in baseline in CD30 positive group was 77 ± 7.88%, in negative group was 76.3 ± 17.78 % (P = 0.292). After 28 days of chemotherapy mean minimal residual disease (MRD) was 1.07 ± 3.754 in the negative group, 0.12 ± 0.034 in the positive group (P = 0.025). Conclusion: Lower MRD on day 28 after chemotherapy was seen in B-ALL patients with baseline CD30 expression.

Keywords

• acute lymphoblastic leukemia
• b lymphoblastic leukemia
• cd30
• flow cytometry
• minimum residual disease