Frontiers in Cell and Developmental Biology (Mar 2022)
Growth Hormone Receptor Controls Adipogenic Differentiation of Chicken Bone Marrow Mesenchymal Stem Cells by Affecting Mitochondrial Biogenesis and Mitochondrial Function
- Changbin Zhao,
- Changbin Zhao,
- Changbin Zhao,
- Changbin Zhao,
- Bowen Hu,
- Bowen Hu,
- Bowen Hu,
- Bowen Hu,
- Zhiying Liao,
- Zhiying Liao,
- Zhiying Liao,
- Zhiying Liao,
- Haohui Wei,
- Haohui Wei,
- Haohui Wei,
- Haohui Wei,
- Yongxia Zhao,
- Yongxia Zhao,
- Yongxia Zhao,
- Yongxia Zhao,
- Jinping Liang,
- Jinping Liang,
- Jinping Liang,
- Jinping Liang,
- Wen Luo,
- Wen Luo,
- Wen Luo,
- Wen Luo,
- Qinghua Nie,
- Qinghua Nie,
- Qinghua Nie,
- Qinghua Nie,
- Qingbin Luo,
- Qingbin Luo,
- Qingbin Luo,
- Qingbin Luo,
- Dexiang Zhang,
- Dexiang Zhang,
- Dexiang Zhang,
- Dexiang Zhang,
- Xiquan Zhang,
- Xiquan Zhang,
- Xiquan Zhang,
- Xiquan Zhang,
- Hongmei Li,
- Hongmei Li,
- Hongmei Li,
- Hongmei Li
Affiliations
- Changbin Zhao
- Lingnan Guangdong Laboratory of Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Changbin Zhao
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science, South China Agricultural University, Guangzhou, China
- Changbin Zhao
- Guangdong Provincial Key Lab of Agro-Animal Genomics, Ministry of Agriculture, Guangzhou, China
- Changbin Zhao
- Molecular Breeding and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, China
- Bowen Hu
- Lingnan Guangdong Laboratory of Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Bowen Hu
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science, South China Agricultural University, Guangzhou, China
- Bowen Hu
- Guangdong Provincial Key Lab of Agro-Animal Genomics, Ministry of Agriculture, Guangzhou, China
- Bowen Hu
- Molecular Breeding and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, China
- Zhiying Liao
- Lingnan Guangdong Laboratory of Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Zhiying Liao
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science, South China Agricultural University, Guangzhou, China
- Zhiying Liao
- Guangdong Provincial Key Lab of Agro-Animal Genomics, Ministry of Agriculture, Guangzhou, China
- Zhiying Liao
- Molecular Breeding and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, China
- Haohui Wei
- Lingnan Guangdong Laboratory of Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Haohui Wei
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science, South China Agricultural University, Guangzhou, China
- Haohui Wei
- Guangdong Provincial Key Lab of Agro-Animal Genomics, Ministry of Agriculture, Guangzhou, China
- Haohui Wei
- Molecular Breeding and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, China
- Yongxia Zhao
- Lingnan Guangdong Laboratory of Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Yongxia Zhao
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science, South China Agricultural University, Guangzhou, China
- Yongxia Zhao
- Guangdong Provincial Key Lab of Agro-Animal Genomics, Ministry of Agriculture, Guangzhou, China
- Yongxia Zhao
- Molecular Breeding and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, China
- Jinping Liang
- Lingnan Guangdong Laboratory of Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Jinping Liang
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science, South China Agricultural University, Guangzhou, China
- Jinping Liang
- Guangdong Provincial Key Lab of Agro-Animal Genomics, Ministry of Agriculture, Guangzhou, China
- Jinping Liang
- Molecular Breeding and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, China
- Wen Luo
- Lingnan Guangdong Laboratory of Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Wen Luo
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science, South China Agricultural University, Guangzhou, China
- Wen Luo
- Guangdong Provincial Key Lab of Agro-Animal Genomics, Ministry of Agriculture, Guangzhou, China
- Wen Luo
- Molecular Breeding and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, China
- Qinghua Nie
- Lingnan Guangdong Laboratory of Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Qinghua Nie
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science, South China Agricultural University, Guangzhou, China
- Qinghua Nie
- Guangdong Provincial Key Lab of Agro-Animal Genomics, Ministry of Agriculture, Guangzhou, China
- Qinghua Nie
- Molecular Breeding and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, China
- Qingbin Luo
- Lingnan Guangdong Laboratory of Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Qingbin Luo
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science, South China Agricultural University, Guangzhou, China
- Qingbin Luo
- Guangdong Provincial Key Lab of Agro-Animal Genomics, Ministry of Agriculture, Guangzhou, China
- Qingbin Luo
- Molecular Breeding and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, China
- Dexiang Zhang
- Lingnan Guangdong Laboratory of Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Dexiang Zhang
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science, South China Agricultural University, Guangzhou, China
- Dexiang Zhang
- Guangdong Provincial Key Lab of Agro-Animal Genomics, Ministry of Agriculture, Guangzhou, China
- Dexiang Zhang
- Molecular Breeding and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, China
- Xiquan Zhang
- Lingnan Guangdong Laboratory of Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Xiquan Zhang
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science, South China Agricultural University, Guangzhou, China
- Xiquan Zhang
- Guangdong Provincial Key Lab of Agro-Animal Genomics, Ministry of Agriculture, Guangzhou, China
- Xiquan Zhang
- Molecular Breeding and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, China
- Hongmei Li
- Lingnan Guangdong Laboratory of Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China
- Hongmei Li
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science, South China Agricultural University, Guangzhou, China
- Hongmei Li
- Guangdong Provincial Key Lab of Agro-Animal Genomics, Ministry of Agriculture, Guangzhou, China
- Hongmei Li
- Molecular Breeding and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, China
- DOI
- https://doi.org/10.3389/fcell.2022.827623
- Journal volume & issue
-
Vol. 10
Abstract
Growth hormone receptor (GHR) can activate several signaling pathways after binding to growth hormone (GH) to regulate cell growth and development. Sex-linked dwarf (SLD) chickens, normal protein functions are prevented because of exon mutations in the GHR gene, have more severe fat deposition. However, the specific molecular mechanisms responsible for this phenotype remains unclear. We therefore investigated the effect of the GHR gene on adipogenic differentiation of chicken bone marrow mesenchymal stem cells (BMSCs). We found that bone marrow fat deposition was more severe in SLD chickens compared to normal chickens, and the expression of genes related to adipogenic differentiation was enhanced in SLD chicken BMSCs. We also detected enhanced mitochondrial function of BMSCs in SLD chickens. In vitro, overexpression of GHR in chicken BMSCs increased mitochondrial membrane potential but decreased reactive oxygen and ATP contents, oxidative phosphorylation complex enzyme activity, and mitochondrial number. Expression of genes associated with mitochondrial biogenesis and function was repressed during adipogenic differentiation in chicken BMSCs, the adipogenic differentiation capacity of chicken BMSCs was also repressed. With knockdown of GHR, opposite results were observed. We concluded that GHR inhibited adipogenic differentiation of chicken BMSCs by suppressing mitochondrial biogenesis and mitochondrial function.
Keywords
- GHR
- bone mesenchymal stem cells
- mitochondrial biogenesis
- mitochondrial function
- adipogenic differentiation
- sex-linked dwarf chickens
