Journal of Nanobiotechnology (Dec 2021)

Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection

  • Hongke Qu,
  • Chunmei Fan,
  • Mingjian Chen,
  • Xiangyan Zhang,
  • Qijia Yan,
  • Yumin Wang,
  • Shanshan Zhang,
  • Zhaojian Gong,
  • Lei Shi,
  • Xiayu Li,
  • Qianjin Liao,
  • Bo Xiang,
  • Ming Zhou,
  • Can Guo,
  • Guiyuan Li,
  • Zhaoyang Zeng,
  • Xu Wu,
  • Wei Xiong

DOI
https://doi.org/10.1186/s12951-021-01149-z
Journal volume & issue
Vol. 19, no. 1
pp. 1 – 28

Abstract

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Abstract The cyclic signal amplification technology has been widely applied for the ultrasensitive detection of many important biomolecules, such as nucleic acids, proteins, enzymes, adenosine triphosphate (ATP), metal ions, exosome, etc. Due to their low content in the complex biological samples, traditional detection methods are insufficient to satisfy the requirements for monitoring those biomolecules. Therefore, effective and sensitive biosensors based on cyclic signal amplification technology are of great significance for the quick and simple diagnosis and treatment of diseases. Fluorescent biosensor based on cyclic signal amplification technology has become a research hotspot due to its simple operation, low cost, short time, high sensitivity and high specificity. This paper introduces several cyclic amplification methods, such as rolling circle amplification (RCA), strand displacement reactions (SDR) and enzyme-assisted amplification (EAA), and summarizes the research progress of using this technology in the detection of different biomolecules in recent years, in order to provide help for the research of more efficient and sensitive detection methods. Graphical Abstract

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