BioTechniques (Feb 2001)

Serum-Free Recombinant Production of Adenovirus Using a Hollow Fiber Capillary System

  • T.A. Gardner,
  • S.-C. Ko,
  • L. Yang,
  • J.J.S. Cadwell,
  • L.W.K. Chung,
  • C. Kao

DOI
https://doi.org/10.2144/01302pf01
Journal volume & issue
Vol. 30, no. 2
pp. 422 – 427

Abstract

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A novel method for the production of adenoviral vectors on a scale sufficient to support most research applications and early phase clinical trials is presented. This method utilizes serum-free cell culture medium and a hollow fiber cell culture apparatus. Significantly less time and space are required than in conventional methods, and the resulting adenovirus is collected in a much smaller volume, simplifying the purification steps. The protocol described is a reproducible, convenient, biologically safe, and environmentally sound method for the production of adenoviral vectors for laboratory use and has the potential to scale-up the adenovirus production for clinical use.