New method of investigation of urine microflora in mice after irradiation

Abstract

For investigation of urine microflora in mice we offered to use paper disks. This new method was tested in finding of infection on the mice (CBAxC57BI) F1 after total body v-irradiation at 6,5 Gr (LD80/30). The paper disks were impreg- nated with one drop of urine, placed on surface Endo media, incubate in thermostat (37°C) in during 1-2 days and numbers of positive crops (Esch. coli, Proteus, Enterococcus, were indicated. It was shown that after irradiation the numbers of positive probes increased. The test displayed that the method is easy, economic and demonstrative.

Keywords

• Endo media
• paper discs
• urine
• y-rays