An efficient transformation method for tannin-containing sorghum
Yuan Liang,
Xuehui Yan,
Jingyi Xu,
Yanlong Liu,
Ke Xie,
Jieqin Li,
Qiuwen Zhan
Affiliations
Yuan Liang
College of Agricultural, Anhui Science and Technology University, Fengyang, Anhui, China
Xuehui Yan
College of Agricultural, Anhui Science and Technology University, Fengyang, Anhui, China
Jingyi Xu
College of Agricultural, Anhui Science and Technology University, Fengyang, Anhui, China
Yanlong Liu
College of Agricultural, Anhui Science and Technology University, Fengyang, Anhui, China
Ke Xie
Research Center of Biology and Agriculture, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China
Jieqin Li
College of Agricultural, Anhui Science and Technology University, Fengyang, Anhui, China
Qiuwen Zhan
College of Agricultural, Anhui Science and Technology University, Fengyang, Anhui, China
Background Tannins are the main bottlenecks restricting the transformation efficiency of plants. Hongyingzi is a special tannin-containing sorghum cultivar used in brewing. Methods In this study, a highly efficient microprojectile transformation system for tannin-containing sorghum was successfully exploited using immature embryos (IEs) of Hongyingzi as explants. Results Hongyingzi presented two types of calli. Type II calli were found to be the most suitable and effective explants for transformation. After optimization of the geneticin (G418) concentration and tissue culture medium, an average transformation frequency of 27% was achieved. Molecular analyzis showed that all transgenic plants were positive and showed transgenes expression. The inheritance analyzis confirmed that the transgenes could be inherited into the next generation. Thus, we successfully established an efficient transformation system for tannin-containing sorghum and demonstrated the possibility of breaking the restriction imposed by tannins in plants.
