Cultivation of saprophytic fungus Trichoderma and biosynthesis of L-lysine-α-oxidase

Abstract

Studies on the growth of the fungus Trichoderma and biosynthesis of L-lysine-α-oxidase enzyme were carried out with surface and deep methods of cultivation. Factors which control the activity of Trichoderma's metabolite L-lysine-α-oxidase were determined: relationship between enzyme's biosynthesis and the fungus sporulation; number of inoculum, aeration; initial pH value of fermentation medium; number of sources of carbon and nitrogen, degree of their assimilation. The possibility of secondary use of substrate for production of enzyme in vitro was indicated.

Keywords

• триходерма
• метаболит
• фермент
• культивирование
• биосинтез