Generation of human induced pluripotent stem cells (NIHTVBi004-A, NIHTVBi005-A, NIHTVBi006-A, NIHTVBi007-A, NIHTVBi008-A) from 5 CADASIL patients with NOTCH3 mutation

Guibin Chen; Zhongwen Li; Yangtengyu Liu; Daniel Chen; Jeanette Beers; Cornelia Cudrici; Elisa A. Ferrante; Robin Schwartzbeck; Natalia Dmitrieva; Dan Yang; Jizhong Zou; M. Luisa Iruela-Arispe; Manfred Boehm

Stem Cell Research (May 2020)

Generation of human induced pluripotent stem cells (NIHTVBi004-A, NIHTVBi005-A, NIHTVBi006-A, NIHTVBi007-A, NIHTVBi008-A) from 5 CADASIL patients with NOTCH3 mutation

Guibin Chen,
Zhongwen Li,
Yangtengyu Liu,
Daniel Chen,
Jeanette Beers,
Cornelia Cudrici,
Elisa A. Ferrante,
Robin Schwartzbeck,
Natalia Dmitrieva,
Dan Yang,
Jizhong Zou,
M. Luisa Iruela-Arispe,
Manfred Boehm

Affiliations

Guibin Chen: Translational Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, United States; Corresponding author.
Zhongwen Li: Translational Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, United States; Guangdong General Hospital, Guangzhou, Guangdong 510080, China
Yangtengyu Liu: Translational Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, United States; Current address: Department of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha, China
Daniel Chen: Translational Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, United States
Jeanette Beers: Induced Pluripotent Stem Cells (iPSC) Core, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, United States
Cornelia Cudrici: Translational Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, United States
Elisa A. Ferrante: Translational Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, United States
Robin Schwartzbeck: Translational Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, United States
Natalia Dmitrieva: Translational Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, United States
Dan Yang: Translational Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, United States
Jizhong Zou: Induced Pluripotent Stem Cells (iPSC) Core, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, United States
M. Luisa Iruela-Arispe: Department of Cell and Developmental Biology, Northwestern University, Feinberg School of Medicine, Chicago, IL, 60611, United States
Manfred Boehm: Translational Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, United States

Journal volume & issue: Vol. 45

Abstract

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We have successfully generated induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells of five patients with Cerebral autosomal-dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). These cells carry the genetic NOTCH3 mutation present in their parental cells. These iPSC cells exhibited normal karyotype and phenotype, which were sustained through propagation. Furthermore, these iPSCs displayed the capacity of differentiating toward the three germ layers in vitro.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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