Effects of different antioxidants on cryopreservation of Jinhua boar sperm

Abstract

To compare the effects of different antioxidant concentrations of glutathione (GSH), green tea polyphenols (GTPs), vitamin E (VE) and superoxide dismutase (SOD) on cryopreservation of Jinhua boar sperm, freezing extenders were supplemented with the GSH (0.125, 0.25 and 0.5 mmol·L-1), GTPs (10, 15 and 20 mmol·L-1), VE (1, 2 and 4 mg·mL-1) and SOD (1 000, 2 000 and 4 000 IU), respectively. The boar sperms were collected and frozen with extenders containing above antioxidants. Sperm motility after 4℃ cooling, sperm motility, membrane integrity and acrosome integrity after thawing were examined in four treatment groups. The results show that sperm motility after 4℃ cooling, sperm motility and membrane integrity after thawing were increased significantly by supplemented boar freezing extenders with 15 mmol·L-1 GTPs, 2 mg·mL-1 VE or 4 000 IU SOD, respectively (P<0.05).

Keywords

• antioxidants
• sperm
• Jinhua boar
• cryoprotective agents