Production of oleic acid ethyl ester catalyzed by crude rice bran (Oryza sativa) lipase in a modified fed-batch system: problem and its solution

Bulletin of Chemical Reaction Engineering & Catalysis. 2015;0(x) DOI 10.9767/


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Journal Title: Bulletin of Chemical Reaction Engineering & Catalysis

ISSN: 1978-2993 (Print)

Publisher: Diponegoro University

Society/Institution: Indonesian Catalyst Society (Masyarakat Katalis Indonesia)

LCC Subject Category: Technology: Chemical technology: Chemical engineering

Country of publisher: Indonesia

Language of fulltext: Indonesian, English

Full-text formats available: PDF



Indro Prastowo (Ahmad Dahlan University)


Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 12 weeks


Abstract | Full Text

<span>A fed-batch system was modified</span><span> </span><span>for the enzymatic production of </span><span lang="EN-GB">Oleic Acid Ethyl Ester (OAEE)</span><span lang="EN-GB"> </span><span lang="EN-GB">using</span><span lang="EN-GB"> </span><span lang="EN-GB">rice bran</span><span lang="EN-GB"> </span><span lang="EN-GB">(<em>Oryza</em></span><em><span lang="EN-GB"> </span><span lang="EN-GB">sativa</span></em><span lang="EN-GB">) lipase</span><span> by retaining the substrate molar ratio (ethanol/oleic acid) </span><span lang="EN-US">at</span><span> 2.05: 1 during the reaction. It </span><span>resulted in an increase in the ester conversion up to 76.8% in the first 6 h of the reaction, and then followed by a decrease </span><span lang="EN-GB">from </span><span>76.8</span><span lang="EN-GB">% to </span><span>22</span><span lang="EN-GB">.</span><span>9</span><span lang="EN-GB">%</span><span> in 6 h later. Meanwhile, the production of water in the reaction system </span><span lang="EN-US">also </span><span>showed a similar trend to the trend of ester production</span><span lang="EN-US">.</span><span lang="EN-US"> </span><span>The water wa</span><span lang="EN-US">s </span><span>hypothesized</span><span> </span><span>to lead lipase to reverse the reaction which resulted in a</span><span> </span><span>decrease in both (water and esters) in the last 6 h of the reaction. </span><span lang="EN-US">In order to overcome the problem,</span><span> zeolite powders (25 and 50 mg/ml) </span><span lang="EN-US">were added </span><span>into the reaction system at 5 h of the reaction</span><span lang="EN-US">. As </span><span>the</span><span lang="EN-US"> result, </span><span>final ester conversions</span><span lang="EN-US"> increased</span><span lang="EN-US"> </span><span>drastically </span><span lang="EN-US">up to</span><span lang="EN-US"> </span><span>90 - 95.7% (1.17 – 1.24 times). The addition also proved a hypothesis that the water was involved in reducing the ester conversion </span><span>in the last 6 h of the reaction. Thus, </span><span lang="EN-US">the</span><span> combination was effective to produce the high final ester conversion.</span>