Expression of hypoxia-inducible factor-1α and erythropoietin at corneal neovascularization in rats

Abstract

AIM: To describe the expression of hypoxia-inducible factor-1α(HIF-1α)and erythropoietin(EPO)in rats' corneal and evaluate its potential effect on corneal neovascularization(CNV)growth. METHODS: The young SD rats(3mo)was chosen and randomly divided into 2 groups, which were experimental group and normal control group. CNV model was established by alkali burn, and the length and area of CNV was observed everyday after operation by slit lamp. After that, the expression of HIF-1α and EPO was measured by SABC and RT-PCR methods at 1, 3, 5, 7, and 14d after alkali burn. The data was analyzed by SPSS 20.0. RESULTS: The area of CNV was increasing at 1, 3, 5, 7, and 14d after alkali burn, and the peak point appear at 7d. The growth speed was decreased after 14d. SABC method told us that no HIF-1α and very tiny amount EPO was detected at normal rats' corneal. The expression of the two factors increased at 1d after alkali burn in corneal epithelium and endoderm. The results of RT-PCR showed that a few amounts of HIF-1α and EPO mRNA were detected at normal group. The expression of the two factors was increased at 3d after alkali burn, and the peak value was found at 7d, however, it was decreased at 14d. Statistical difference was found at different time(PCONCLUSION: HIF-1α and EPO is closely related to CNV.

Keywords

• corneal neovascularization
• alkali burn
• hypoxia-inducible factor-1α
• erythropoietin