Development of Antibody-Coated Magnetite Nanoparticles for Biomarker Immobilization

Journal of Nanomaterials. 2014;2014 DOI 10.1155/2014/978284

 

Journal Homepage

Journal Title: Journal of Nanomaterials

ISSN: 1687-4110 (Print); 1687-4129 (Online)

Publisher: Hindawi Publishing Corporation

LCC Subject Category: Technology: Technology (General)

Country of publisher: Egypt

Language of fulltext: English

Full-text formats available: PDF, HTML, ePUB

 

AUTHORS

Christian Chapa Gonzalez (Universidad Autónoma de Ciudad Juárez, Instituto de Ingeniería y Tecnología, Avenida del Charro 610 Norte, Colonia Partido Romero, 32315 Ciudad Juárez, CHIH, Mexico)
Carlos A. Martínez Pérez (Universidad Autónoma de Ciudad Juárez, Instituto de Ingeniería y Tecnología, Avenida del Charro 610 Norte, Colonia Partido Romero, 32315 Ciudad Juárez, CHIH, Mexico)
Alejandro Martínez Martínez (Universidad Autónoma de Ciudad Juárez, Instituto de Ingeniería y Tecnología, Avenida del Charro 610 Norte, Colonia Partido Romero, 32315 Ciudad Juárez, CHIH, Mexico)
Imelda Olivas Armendáriz (Universidad Autónoma de Ciudad Juárez, Instituto de Ingeniería y Tecnología, Avenida del Charro 610 Norte, Colonia Partido Romero, 32315 Ciudad Juárez, CHIH, Mexico)
Oscar Zavala Tapia (Universidad Autónoma de Ciudad Juárez, Instituto de Ingeniería y Tecnología, Avenida del Charro 610 Norte, Colonia Partido Romero, 32315 Ciudad Juárez, CHIH, Mexico)
Adriana Martel-Estrada (Universidad Autónoma de Ciudad Juárez, Instituto de Ingeniería y Tecnología, Avenida del Charro 610 Norte, Colonia Partido Romero, 32315 Ciudad Juárez, CHIH, Mexico)
Perla E. García-Casillas (Universidad Autónoma de Ciudad Juárez, Instituto de Ingeniería y Tecnología, Avenida del Charro 610 Norte, Colonia Partido Romero, 32315 Ciudad Juárez, CHIH, Mexico)

EDITORIAL INFORMATION

Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 16 weeks

 

Abstract | Full Text

Magnetic nanoparticles (MNPs) have great potential in biomedical applications because of their magnetic response offers the possibility to direct them to specific areas and target biological entities. Magnetic separation of biomolecules is one of the most important applications of MNPs because their versatility in detecting cancer biomarkers. However, the effectiveness of this method depends on many factors, including the type of functionalization onto MNPs. Therefore, in this study, magnetite nanoparticles have been developed in order to separate the 5′-nucleotidase enzyme (5eNT). The 5eNT is used as a bio-indicator for diagnosing diseases such as hepatic ischaemia, liver tumor, and hepatotoxic drugs damage. Magnetic nanoparticles were covered in a core/shell type with silica, aminosilane, and a double shell of silica-aminosilane. A ScFv (fragment antibody) and anti-CD73 antibody were attached to the coated nanoparticles in order to separate the enzyme. The magnetic separation of this enzyme with fragment antibody was found to be 28% higher than anti-CD73 antibody and the enzyme adsorption was improved with the double shell due to the increased length of the polymeric chain. Magnetite nanoparticles with a double shell (silica-aminosilane) were also found to be more sensitive than magnetite with a single shell in the detection of biomarkers.