Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery

Pengwei Wang; Roman Pleskot; Jingze Zang; Joanna Winkler; Jie Wang; Klaas Yperman; Tong Zhang; Kun Wang; Jinli Gong; Yajie Guan; Christine Richardson; Patrick Duckney; Michael Vandorpe; Evelien Mylle; Jindriska Fiserova; Daniel Van Damme; Patrick J. Hussey

doi:10.1038/s41467-019-12782-6

Nature Communications (Nov 2019)

Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery

Pengwei Wang,
Roman Pleskot,
Jingze Zang,
Joanna Winkler,
Jie Wang,
Klaas Yperman,
Tong Zhang,
Kun Wang,
Jinli Gong,
Yajie Guan,
Christine Richardson,
Patrick Duckney,
Michael Vandorpe,
Evelien Mylle,
Jindriska Fiserova,
Daniel Van Damme,
Patrick J. Hussey

Affiliations

Pengwei Wang: Department of Biosciences, Durham University
Roman Pleskot: Department of Plant Biotechnology and Bioinformatics, Ghent University
Jingze Zang: Department of Biosciences, Durham University
Joanna Winkler: Department of Plant Biotechnology and Bioinformatics, Ghent University
Jie Wang: Department of Plant Biotechnology and Bioinformatics, Ghent University
Klaas Yperman: Department of Plant Biotechnology and Bioinformatics, Ghent University
Tong Zhang: Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University
Kun Wang: Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University
Jinli Gong: Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University
Yajie Guan: Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University
Christine Richardson: Department of Biosciences, Durham University
Patrick Duckney: Department of Biosciences, Durham University
Michael Vandorpe: Department of Plant Biotechnology and Bioinformatics, Ghent University
Evelien Mylle: Department of Plant Biotechnology and Bioinformatics, Ghent University
Jindriska Fiserova: Department of Biosciences, Durham University
Daniel Van Damme: Department of Plant Biotechnology and Bioinformatics, Ghent University
Patrick J. Hussey: Department of Biosciences, Durham University

DOI: https://doi.org/10.1038/s41467-019-12782-6
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 16

Abstract

Read online

Arabidopsis EH/Pan1 proteins are part of the TPLATE complex (TPC) that is required for endocytosis in plants. Here, the authors show AtEH/Pan1 proteins also act in actin-mediated autophagy, by interacting with VAP27-1 at ER-PM contact sites and recruiting TPLATE and AP-2 complex subunits, clathrin and ARP2/3/ proteins to autophagosomes.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal