Layer-by-Layer Assembly of Biopolyelectrolytes onto Thermo/pH-Responsive Micro/Nano-Gels

Materials. 2014;7(11):7472-7512 DOI 10.3390/ma7117472


Journal Homepage

Journal Title: Materials

ISSN: 1996-1944 (Print)

Publisher: MDPI AG

LCC Subject Category: Technology: Electrical engineering. Electronics. Nuclear engineering | Technology: Engineering (General). Civil engineering (General) | Science: Natural history (General): Microscopy | Science: Physics: Descriptive and experimental mechanics

Country of publisher: Switzerland

Language of fulltext: English

Full-text formats available: PDF, HTML



Ana M. Díez-Pascual (Analytical Chemistry, Physical Chemistry and Chemical Engineering Department, Faculty of Biology, Environmental Sciences and Chemistry, Alcalá University, 28871 Alcalá de Henares, Madrid, Spain)
Peter S. Shuttleworth (Instituto de Ciencia y Tecnología de Polímeros-Consejo Superior de Investigaciones Científicas, Juan de la Cierva 3, 28006 Madrid, Spain)


Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 11 weeks


Abstract | Full Text

This review deals with the layer-by-layer (LbL) assembly of polyelectrolyte multilayers of biopolymers, polypeptides (i.e., poly-l-lysine/poly-l-glutamic acid) and polysaccharides (i.e., chitosan/dextran sulphate/sodium alginate), onto thermo- and/or pH-responsive micro- and nano-gels such as those based on synthetic poly(N-isopropylacrylamide) (PNIPAM) and poly(acrylic acid) (PAA) or biodegradable hyaluronic acid (HA) and dextran-hydroxyethyl methacrylate (DEX-HEMA). The synthesis of the ensembles and their characterization by way of various techniques is described. The morphology, hydrodynamic size, surface charge density, bilayer thickness, stability over time and mechanical properties of the systems are discussed. Further, the mechanisms of interaction between biopolymers and gels are analysed. Results demonstrate that the structure and properties of biocompatible multilayer films can be finely tuned by confinement onto stimuli-responsive gels, which thus provides new perspectives for biomedical applications, particularly in the controlled release of biomolecules, bio-sensors, gene delivery, tissue engineering and storage.