Science Journal of University of Zakho (Sep 2013)
Antibacterial Bioactive Compound From The Fungus Drechslera Halodes (Drechsler) Subram. & Jain Isolated From Soil of Basrah, Iraq
Abstract
The fungus Drechslera halodes was isolated from soil samples of Basrah, Iraq, and examined for its ability to produce antimicrobial bioactive compounds after being cultured in two types of liquid media for a production of secondary metabolites. The fungal culture filtrate and mycelia extracts were tested against two strains of bacteria E. coli and S. aureus using a disc diffusion method. The fungal filtrate exhibited a growth inhibition against both bacteria E. coli and S. aureus with inhibition zones of 25 mm and 30 mm diam., respectively. The minimal inhibitory concentrations (MIC) of the purified compound was examined and revealed that 50 ug/ml was against E. coli and 25 ug/ml for S. aureus. Toxicity of the fungal extract using human blood indicating that no toxic effect was detected. The fungal extract revealed three separated compounds by using thin layer chromatography (TLC). Solubility, melting points and functional chemical groups were also determined. A bioactive compound was isolated, purified and identified by using Gas Chromatography (GC-mass) and Infra Red (IR). The bioactive compound is identified as 6-allyl-5,6-dihydro-5-hydroxypyran-2-one, with a molecular weight of 154 kd and the chemical formula is C8H10O3 which belongs to aromatic esterase group.
