High-Throughput Sequencing Reveals Three Rhabdoviruses Persisting in the IRE/CTVM19 Cell Line
Alexander G. Litov,
Alexey M. Shchetinin,
Ivan S. Kholodilov,
Oxana A. Belova,
Magomed N. Gadzhikurbanov,
Anna Y. Ivannikova,
Anastasia A. Kovpak,
Vladimir A. Gushchin,
Galina G. Karganova
Affiliations
Alexander G. Litov
Laboratory of Biology of Arboviruses, FSASI “Chumakov FSC R&D IBP RAS” (Institute of Poliomyelitis), 108819 Moscow, Russia
Alexey M. Shchetinin
Pathogenic Microorganisms Variability Laboratory, Gamaleya Federal Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, Russia
Ivan S. Kholodilov
Laboratory of Biology of Arboviruses, FSASI “Chumakov FSC R&D IBP RAS” (Institute of Poliomyelitis), 108819 Moscow, Russia
Oxana A. Belova
Laboratory of Biology of Arboviruses, FSASI “Chumakov FSC R&D IBP RAS” (Institute of Poliomyelitis), 108819 Moscow, Russia
Magomed N. Gadzhikurbanov
Laboratory of Biology of Arboviruses, FSASI “Chumakov FSC R&D IBP RAS” (Institute of Poliomyelitis), 108819 Moscow, Russia
Anna Y. Ivannikova
Laboratory of Biology of Arboviruses, FSASI “Chumakov FSC R&D IBP RAS” (Institute of Poliomyelitis), 108819 Moscow, Russia
Anastasia A. Kovpak
Laboratory of Biochemistry, FSASI “Chumakov FSC R&D IBP RAS” (Institute of Poliomyelitis), 108819 Moscow, Russia
Vladimir A. Gushchin
Pathogenic Microorganisms Variability Laboratory, Gamaleya Federal Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, Russia
Galina G. Karganova
Laboratory of Biology of Arboviruses, FSASI “Chumakov FSC R&D IBP RAS” (Institute of Poliomyelitis), 108819 Moscow, Russia
Cell cultures derived from ticks have become a commonly used tool for the isolation and study of tick-borne pathogens and tick biology. The IRE/CTVM19 cell line, originating from embryos of Ixodes ricinus, is one such line. Previously, reovirus-like particles, as well as sequences with similarity to rhabdoviruses and iflaviruses, were detected in the IRE/CTVM19 cell line, suggesting the presence of multiple persisting viruses. Subsequently, the full genome of an IRE/CTVM19-associated rhabdovirus was recovered from a cell culture during the isolation of the Alongshan virus. In the current work, we used high-throughput sequencing to describe a virome of the IRE/CTVM19 cell line. In addition to the previously detected IRE/CTVM19-associated rhabdovirus, two rhabdoviruses were detected: Chimay rhabdovirus and Norway mononegavirus 1. In the follow-up experiments, we were able to detect both positive and negative RNA strands of the IRE/CTVM19-associated rhabdovirus and Norway mononegavirus 1 in the IRE/CTVM19 cells, suggesting their active replication in the cell line. Passaging attempts in cell lines of mammalian origin failed for all three discovered rhabdoviruses.
