The usage of smart glasses in goods logistics and order picking has mainly been studied through cross-sectional experimental studies. Our longitudinal field study investigated the effects of smart glasses on the eyesight of 43 employees at two German companies. We combined ophthalmological examinations and questionnaire surveys at two points in time, six months apart. The vision of the employees was examined before and after each work shift. Mixed effects logistic regression was conducted to determine the associations between smart glasses use and effects on visual acuity. In the baseline examination, differences in eyesight before and after shifts were small and not statistically significant. However, some individuals experienced deteriorations, especially in visual acuity at near distances (n = 7 for the right eye, n = 6 for the left). Participants over 40 years of age had 16.1 times higher odds of deterioration compared to those under 40 years (95% CI: 2.7–95.9, p = 0.002). The most commonly reported eye strains were eye fatigue (n = 32), rubbing (n = 25), and burning (n = 24). If smart glasses are to be implemented in logistics companies, it is recommended to offer employees eye tests with an industrial physician in advance.