Molecular Mechanisms of Intracellular Delivery of Nanoparticles Monitored by an Enzyme-Induced Proximity Labeling

Abstract

Highlights Novel enzyme-induced proximity labeling technology in nanoparticles (nano-EPL). Nano-EPL enables dynamic molecular mapping of the intracellular delivery of nanoparticles in macrophages. Nano-EPL enables the elucidation of a comprehensive phagosome-centered timeline during the vesicular transportation of nanoparticles, revealing distinct organelle engagement and its differential impact on drug delivery efficiency.

Keywords

• Enzyme-induced proximity labeling
• Intracellular delivery
• Nano-protein interaction
• Dynamic molecule profiling
• Macrophages